Nitric Oxide Plays a Central Role in Water Stress-Induced Tanshinone Production in Salvia miltiorrhiza Hairy Roots

Molecules

Xuhong Du³  Chenlu Zhang²  Wanli Guo³  Weibo Jin³  Zongsuo Liang³  Xijun Yan¹  Zhixin Guo¹  Yan Liu⁴  Dongfeng Yang³ 

[1] Tasly R&D Institute, Tasly Holding Group Co. Ltd, Tianjin 300410, China; E-Mails:;College of Biological Science & Engineering, Shaanxi University of Technology, Hanzhong 723000, China; E-Mail:;College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China; E-Mails:;Tianjin Tasly Modern TCM Resources Co., Ltd., Tianjin 300402, China; E-Mail:

关键词: nitric oxide;    tanshinone;    Salvia miltiorrhiza;    polyethylene glycol;    abscisic acid;   

DOI  :  10.3390/molecules20057574

来源: mdpi

【 摘 要 】

Nitric oxide (NO), a well-known signaling molecule plays an important role in abiotic and biotic stress-induced production of plant secondary metabolites. In this study, roles of NO in water stress-induced tanshinone production in Salvia miltiorrhiza hairy roots were investigated. The results showed that accumulations of four tanshinone compounds in S. miltiorrhiza hairy roots were significantly stimulated by sodium nitroprusside (SNP, a NO donor) at 100 μM. Effects of SNP were just partially arrested by the mevalonate (MVA) pathway inhibitor (mevinolin), but were completely inhibited by the 2-C-methyl-d-erythritol-4-phosphate pathway (MEP) inhibitor (fosmidomycin). The increase of tanshinone accumulation and the up-regulation of HMGR and DXR expression by PEG and ABA treatments were partially inhibited by an inhibitor of NO biosynthesis (N^ω-nitro-L-arginine methyl ester (L-NAME)) and a NO scavenger (2-(4-Carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (c-PTIO)). Simultaneously, NO generation in the hairy roots was triggered by PEG and ABA, and the effects were also arrested by c-PTIO and L-NAME. These results indicated that NO signaling probably plays a central role in water stress-induced tanshinone production in S. miltiorrhiza hairy roots. SNP mainly stimulated the MEP pathway to increase tanshinone accumulation.

【 授权许可】

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