Anti-Inflammatory and Analgesic Effects of the Marine-Derived Compound Excavatolide B Isolated from the Culture-Type Formosan Gorgonian Briareum excavatum

Marine Drugs

Yen-You Lin³  Sung-Chun Lin²  Chien-Wei Feng¹  Pei-Chin Chen¹  Yin-Di Su³  Chi-Min Li³  San-Nan Yang⁴  Yen-Hsuan Jean²  Ping-Jyun Sung⁵  Chang-Yih Duh³  Zhi-Hong Wen³ 

[1] Doctoral Degree Program in Marine Biotechnology, National Sun Yat-sen University and Academia Sinica, Kaohsiung 80424, Taiwan; E-Mails:;Department of Orthopaedic Surgery, Ping-Tung Christian Hospital, Ping-Tung 90059, Taiwan; E-Mails:;Department of Marine Biotechnology and Resources, Asia-Pacific Ocean Research Center, National Sun Yat-sen University, Kaohsiung 80424, Taiwan; E-Mails:;School of Medicine, College of Medicine and Department of Pediatrics, E-DA Hospital, I-Shou University, Kaohsiung 84001, Taiwan; E-Mail:;National Museum of Marine Biology & Aquarium, Pingtung 94450, Taiwan; E-Mail:

关键词: excavatolide B;    carrageenan;    lipopolysaccharide;    inducible nitric oxide synthase (iNOS);    cycloxygenase-2 (COX-2);    anti-inflammatory;   

DOI  :  10.3390/md13052559

来源: mdpi

【 摘 要 】

In recent years, several marine-derived compounds have been clinically evaluated. Diterpenes are secondary metabolites from soft coral that exhibit anti-inflammatory, anti-tumor and cytotoxic activities. In the present study, we isolated a natural diterpene product, excavatolide B, from cultured Formosan gorgonian Briareum excavatum and investigated its anti-inflammatory activities. We found that excavatolide B significantly inhibited the mRNA expression of the proinflammatory mediators, inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), in lipopolysaccharide (LPS)-challenged murine macrophages (RAW 264.7). We also examined the anti-inflammatory and anti-nociceptive effects of excavatolide B on intraplantar carrageenan-induced inflammatory responses. Excavatolide B was found to significantly attenuate carrageenan-induced nociceptive behaviors, mechanical allodynia, thermal hyperalgesia, weight bearing deficits and paw edema. In addition, excavatolide B inhibited iNOS, as well as the infiltration of immune cells in carrageenan-induced inflammatory paw tissue.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.