Anti-Inflammatory and Analgesic Effects of the Marine-Derived Compound Comaparvin Isolated from the Crinoid Comanthus bennetti

Molecules

Li-Chai Chen¹  Yen-You Lin¹  Yen-Hsuan Jean³  Yi Lu¹  Wu-Fu Chen²  San-Nan Yang⁶  Hui-Min David Wang⁴  Ing-Yang Jang¹  I-Ming Chen¹  Jui-Hsin Su⁵  Ping-Jyun Sung⁵  Jyh-Horng Sheu¹ 

[1] Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 80424, Taiwan; E-Mails:;Department of Neurosurgery, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung 83301, Taiwan; E-Mail:;Section of Orthopedic Surgery, Pingtung Christian Hospital, Pingtung 90059, Taiwan; E-Mail:;Department of Fragrance and Cosmetic Science, Center of Excellence for Environmental Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan; E-Mail:;Graduate Institute of Marine Biotechnology, National Dong Hwa University, Pingtung 94450, Taiwan; E-Mails:;School of Medicine, College of Medicine and Department of Pediatrics, E-DA Hospital, I-Shou University, Kaohsiung 84001, Taiwan; E-Mail:

关键词: comaparvin;    crinoids;    Comanthus bennetti;    inducible nitric oxide synthase;    lipopolysaccharide;    carrageenan;   

DOI  :  10.3390/molecules190914667

来源: mdpi

【 摘 要 】

To date, no study has been conducted to explore the bioactivity of the crinoid Comanthus bennetti. Here we report the anti-inflammatory properties of comaparvin (5,8-dihydroxy-10-methoxy-2-propylbenzo[h]chromen-4-one) based on in vivo experiments. Our preliminary screening for anti-inflammatory activity revealed that the crude extract of Comanthus bennetti significantly inhibited the expression of pro-inflammatory proteins in lipopolysaccharide (LPS)-stimulated murine RAW 264.7 macrophage cells. Comaparvin isolated from crinoids significantly decreased the expression of inducible nitric oxide synthase (iNOS) protein and mRNA in LPS-stimulated macrophage cells. Moreover, our results showed that post-treatment with comaparvin significantly inhibited mechanical allodynia, thermal hyperalgesia and weight-bearing deficits in rats with carrageenan-induced inflammation. Comaparvin also attenuated leukocyte infiltration and iNOS protein expression in carrageenan-induced inflamed paws. These results suggest that comaparvin is a potential anti-inflammatory therapeutic agent against inflammatory pain.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.