Extracts of Renealmia alpinia (Rottb.) MAAS Protect against Lethality and Systemic Hemorrhage Induced by Bothrops asper Venom: Insights from a Model with Extract Administration before Venom Injection

Toxins

Arley Camilo Patiño¹  Juan Carlos Quintana³  José Mar໚ Gutiérrez²  Alexandra Rucavado²  Dora Mar໚ Benjumea¹  Jaime Andrés Perea༞z¹ 

[1] Food and Pharmaceutical Sciences Faculty, Pharmacy Department, University of Antioquia UdeA, Street 70 N° 52-21, Medellín 050010, Colombia; E-Mails:;Clodomiro Picado Institute, Microbiology Faculty, University of Costa Rica, Dulce Nombre, Vázquez de Coronado, San José 11103, Costa Rica; E-Mails:;School of Medicine, Universidad Cooperativa de Colombia, Sede Medellín, Street 50 A N° 41-20, Medellín 050010, Colombia; E-Mail:

关键词: Renealmia alpinia;    snake venoms;    metalloproteinase;    serine proteinase;    anti-venom activity;    micropropagated plants;   

DOI  :  10.3390/toxins7051532

来源: mdpi

【 摘 要 】

Renealmia alpinia (Rottb.) MAAS, obtained by micropropagation (in vitro) and wild forms have previously been shown to inhibit some toxic activities of Bothrops asper snake venom if preincubated before injection. In this study, assays were performed in a murine model in which extracts were administered for three days before venom injection. R. alpinia extracts inhibited lethal activity of B. asper venom injected by intraperitoneal route. Median Effective Dose (ED₅₀) values were 36.6 ± 3.2 mg/kg and 31.7 ± 5.4 mg/kg (p > 0.05) for R. alpinia wild and in vitro extracts, respectively. At a dose of 75 mg/kg, both extracts totally inhibited the lethal activity of the venom. Moreover, this dose prolonged survival time of mice receiving a lethal dose of venom by the intravenous route. At 75 mg/kg, both extracts of R. alpinia reduced the extent of venom-induced pulmonary hemorrhage by 48.0% (in vitro extract) and 34.7% (wild extract), in agreement with histological observations of lung tissue. R. alpinia extracts also inhibited hemorrhage in heart and kidneys, as evidenced by a decrease in mg of hemoglobin/g of organ. These results suggest the possibility of using R. alpinia as a prophylactic agent in snakebite, a hypothesis that needs to be further explored.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.