Gene Silencing of a Cubilin-Related Serine Proteinase from the Hard Tick Haemaphysalis longicornis by RNA Interference

Journal of Veterinary Medical Science

Takeharu MIYOSHI¹  Naotoshi TSUJI¹  Kozo FUJISAKI²  M. Khyrul ISLAM¹  Tsugihiko KAMIO¹ 

[1] National Institute of Animal Health, National Agricultural Research Organization;National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine

关键词: Haemaphysalis longicornis;    RNA interference;    serine proteinase;   

DOI  :  10.1292/jvms.66.1471

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(21)Cited-By(27)RNA interference (RNAi) has been recently exploited to determine gene function by degrading specific mRNAs in several eukaryotic organisms. We constructed a double stranded RNA (dsRNA) from a previously cloned Haemaphysalis longicornis serine proteinase (HlSP) gene to test the importance of the function of the HlSP gene product during blood-feeding. Growth of unfed ticks treated with HlSP dsRNA was significantly inhibited compared to that of PBS-treated ticks. This inhibition was supported by the level of HlSP mRNA. HlSP may play a crucial role for blood-feeding in these ticks. This is the first report on gene silencing of a functional serine proteinase in hard ticks.

【 授权许可】

Unknown