Journal of Veterinary Medical Science | |
Gene Silencing of a Cubilin-Related Serine Proteinase from the Hard Tick Haemaphysalis longicornis by RNA Interference | |
Takeharu MIYOSHI1  Naotoshi TSUJI1  Kozo FUJISAKI2  M. Khyrul ISLAM1  Tsugihiko KAMIO1  | |
[1] National Institute of Animal Health, National Agricultural Research Organization;National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine | |
关键词: Haemaphysalis longicornis; RNA interference; serine proteinase; | |
DOI : 10.1292/jvms.66.1471 | |
学科分类:兽医学 | |
来源: Japanese Society of Veterinary Science | |
【 摘 要 】
References(21)Cited-By(27)RNA interference (RNAi) has been recently exploited to determine gene function by degrading specific mRNAs in several eukaryotic organisms. We constructed a double stranded RNA (dsRNA) from a previously cloned Haemaphysalis longicornis serine proteinase (HlSP) gene to test the importance of the function of the HlSP gene product during blood-feeding. Growth of unfed ticks treated with HlSP dsRNA was significantly inhibited compared to that of PBS-treated ticks. This inhibition was supported by the level of HlSP mRNA. HlSP may play a crucial role for blood-feeding in these ticks. This is the first report on gene silencing of a functional serine proteinase in hard ticks.
【 授权许可】
Unknown
【 预 览 】
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RO201911300854579ZK.pdf | 44KB | download |