Adventitial Tertiary Lymphoid Organs as Potential Source of MicroRNA Biomarkers for Abdominal Aortic Aneurysm

International Journal of Molecular Sciences

Rafaelle Spear⁵  Ludovic Boytard⁵  Renaud Blervaque⁶  Maggy Chwastyniak⁵  David Hot⁶  Jonathan Vanhoutte³  Bart Staels³  Yves Lemoine⁶  Nicolas Lamblin⁵  François-René Pruvot²  Stephan Haulon¹  Philippe Amouyel⁵  Florence Pinet⁵  Jonathan Golledge⁴ 

[1] INSERM U1008, Lille North of France University, Lille Regional University Hospital, F-59000 Lille, France; E-Mail:;Lille Regional University Hospital, F-59037 Lille, France; E-Mail:;INSERM U1011, Lille Pasteur Institute, Lille North of France University, F-59000 Lille, France; E-Mails:National Institute of Health and Medical Research (INSERM) U1167, Lille Pasteur Institute, Lille North of France University, F-59000 Lille, France;;National Institute of Health and Medical Research (INSERM) U1167, Lille Pasteur Institute, Lille North of France University, F-59000 Lille, France; E-Mails:;INSERM U1019, National Center of Scientific Research (CNRS) Join Research Unit (UMR) 8204, Lille Pasteur Institute, Lille North of France University, F-59000 Lille, France; E-Mails:

关键词: abdominal aortic aneurysm;    adventitial tertiary lymphoid organs;    microRNA;    laser microdissection;    quantitative RT-PCR;   

DOI  :  10.3390/ijms160511276

来源: mdpi

【 摘 要 】

Abdominal aortic aneurysm (AAA) is an inflammatory disease associated with marked changes in the cellular composition of the aortic wall. This study aims to identify microRNA (miRNA) expression in aneurysmal inflammatory cells isolated by laser microdissection from human tissue samples. The distribution of inflammatory cells (neutrophils, B and T lymphocytes, mast cells) was evaluated in human AAA biopsies. We observed in half of the samples that adventitial tertiary lymphoid organs (ATLOs) with a thickness from 0.5 to 2 mm were located exclusively in the adventitia. Out of the 850 miRNA that were screened by microarray in isolated ATLOs (n = 2), 164 miRNAs were detected in ATLOs. The three miRNAs (miR-15a-3p, miR-30a-5p and miR-489-3p) with the highest expression levels were chosen and their expression quantified by RT-PCR in isolated ATLOs (n = 4), M1 (n = 2) and M2 macrophages (n = 2) and entire aneurysmal biopsies (n = 3). Except for the miR-30a-5p, a similar modulation was found in ATLOs and the two subtypes of macrophages. The modulated miRNAs were then evaluated in the plasma of AAA patients for their potential as AAA biomarkers. Our data emphasize the potential of miR-15a-3p and miR-30a-5p as biomarkers of AAA but also as triggers of ATLO evolution. Further investigations will be required to evaluate their targets in order to better understand AAA pathophysiology.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.