Comparison of Aryl Hydrocarbon Receptor Gene Expression in Laser Dissected Granulosa Cell Layers of Immature Rat Ovaries

Journal of Veterinary Medical Science

Yosuke SAKURADA¹  Kaoru INOUE¹  Mariko SHIROTA¹  Masayoshi SAWAI¹  Kinji SHIROTA¹ 

[1] Research Institute of Biosciences and High-Tech Research Center, Azabu University

关键词: aryl hydrocarbon receptor (AhR);    β-subunit of inhibin A;    follicular development;    laser microdissection;    ovary;   

DOI  :  10.1292/jvms.10-0558

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(18)Cited-By(3)In order to understand ovarian toxicity of aryl hydrocarbon receptor (AhR) agonists, in situ gene expression of the AhR was examined during follicle development in immature rats. In situ hybridization on frozen sections of ovaries from 24-day-old Sprague-Dawley rats showed that the AhR mRNA was localized in the granulosa cells and occasionally in the theca cells of the follicles irrespective of the developmental stage. In situ gene quantification on granulosa cell layers collected by laser microdissection further revealed that the granulosa cells expressed less AhR mRNA according to development of belonging follicles, but more β-subunit of inhibin A mRNA, a quality control gene. These results may help to elucidate vulnerable developmental stages of follicles to toxicities of the AhR agonists.

【 授权许可】

Unknown