The Composites of Graphene Oxide with Metal or Semimetal Nanoparticles and Their Effect on Pathogenic Microorganisms

Materials

Lukas Richtera¹  Dagmar Chudobova¹  Kristyna Cihalova¹  Monika Kremplova¹  Vedran Milosavljevic¹  Pavel Kopel¹  Iva Blazkova¹  David Hynek¹  Vojtech Adam¹  Rene Kizek¹ 

[1] Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic; E-Mails:

关键词: Escherichia coli;    graphene oxide;    metal or semimetal based nanoparticles;    Staphylococcus aureus;    antibacterial effect;   

DOI  :  10.3390/ma8062994

来源: mdpi

【 摘 要 】

The present experiment describes a synthesis process of composites based on graphene oxide, which was tested as a carrier for composites of metal- or metalloid-based nanoparticles (Cu, Zn, Mn, Ag, AgP, Se) and subsequently examined as an antimicrobial agent for some bacterial strains (Staphylococcus aureus (S. aureus), methicillin-resistant Staphylococcus aureus (MRSA) and Escherichia coli (E. coli). The composites were first applied at a concentration of 300 µM on all types of model organisms and their effect was observed by spectrophotometric analysis, which showed a decrease in absorbance values in comparison with the control, untreated strain. The most pronounced inhibition (87.4%) of S. aureus growth was observed after the application of graphene oxide composite with selenium nanoparticles compared to control. Moreover, the application of the composite with silver and silver phosphate nanoparticles showed the decrease of 68.8% and 56.8%, respectively. For all the tested composites, the observed antimicrobial effect was found in the range of 26% to 87.4%. Interestingly, the effects of the composites with selenium nanoparticles significantly differed in Gram-positive (G⁺) and Gram-negative (G⁻) bacteria. The effects of composites on bacterial cultures of S. aureus and MRSA, the representatives of G⁺ bacteria, increased with increasing concentrations. On the other hand, the effects of the same composites on G⁻ bacteria E. coli was observed only in the highest applied concentration.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.