Surface Plasmon Resonance (SPR) for the Evaluation of Shear-Force-Dependent Bacterial Adhesion

Biosensors

Oleksandr Zagorodko¹  Julie Bouckaert²  Tetiana Dumych²  Rostyslav Bilyy³  Iban Larroulet⁶  Aritz Yanguas Serrano⁶  Dimitri Alvarez Dorta⁴  Sebastien G. Gouin⁴  Stefan-Ovidiu Dima¹  Florin Oancea⁵  Rabah Boukherroub¹  Sabine Szunerits¹  Nicole Jaffrezic-Renault⁷ 

[1] Institute of Electronics, Microelectronics and Nanotechnology (IEMN), UMR-CNRS 8520, Université Lille 1, Cité Scientifique, 59655 Villeneuve d’Ascq, France; E-Mails:;Unité de Glycobiologie Structurale et Fonctionnelle (UGSF), UMR8576 du CNRS, Université Lille 1, 59655 Villeneuve d’Ascq, France; E-Mail:;Institute of Cell Biology, National Academy of Sciences of Ukraine, 79005 Lviv, Ukraine; E-Mail:;LUNAM Université, CEISAM, UMR 6230 du CNRS, 2, rue de la Houssinière, BP 92208, 44322 Nantes Cedex 3, France; E-Mails:;National R&D Institute for Chemistry and Petrochemistry ICECHIM, 202 Splaiul Independentei, 060021 Bucharest, Romania; E-Mail:;SENSIA SL, Poligono Aranguren, 9, Apdo. Correos 171, 20180 Oiartzun, Gipuzkoa, Spain; E-Mails:Institute of Electronics, Microelectronics and Nanotechnology (IEMN), UMR-CNRS 8520, Université Lille 1, Cité Scientifique, 59655 Villeneuve d’Ascq, France;

关键词: surface plasmon resonance (SPR);    shear force enhancement;    flow rate;    Escherichia coli (E. coli);    carbohydrates;   

DOI  :  10.3390/bios5020276

来源: mdpi

【 摘 要 】

The colonization of Escherichia coli (E. coli) to host cell surfaces is known to be a glycan-specific process that can be modulated by shear stress. In this work we investigate whether flow rate changes in microchannels integrated on surface plasmon resonance (SPR) surfaces would allow for investigating such processes in an easy and high-throughput manner. We demonstrate that adhesion of uropathogenic E. coli UTI89 on heptyl α-d-mannopyranoside-modified gold SPR substrates is minimal under almost static conditions (flow rates of 10 µL·min⁻¹), and reaches a maximum at flow rates of 30 µL·min⁻¹ (≈30 mPa). This concept is applicable to the investigation of any ligand-pathogen interactions, offering a robust, easy, and fast method for screening adhesion characteristics of pathogens to ligand-modified interfaces.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.