Molecular Recognition of Arginine by Supramolecular Complexation with Calixarene Crown Ether Based on Surface Plasmon Resonance

International Journal of Molecular Sciences

Hongxia Chen¹  Limin Gu²  Yongmei Yin¹  Kwangnak Koh³ 

[1] School of Life Sciences, Shanghai University, Shanghai 200444, China; E-Mail:;Henan Chemical Industry Research Institute Co. Ltd., Zhengzhou 450052, China; E-Mail:;College of Nanoscience and Nanotechnology, Pusan National University, Pusan 609-735, Korea; E-Mails:

关键词: arginine;    calixarene crown ether;    amino acid;    self-assembled monolayer (SAM);    surface plasmon resonance (SPR);   

DOI  :  10.3390/ijms12042315

来源: mdpi

【 摘 要 】

Arginine plays an important role in cell division and the functioning of the immune system. We describe a novel method by which arginine can be identified using an artificial monolayer based on surface plasmon resonance (SPR). The affinity of arginine binding its recognition molecular was compared to that of lysine. In fabrication of an arginine sensing interface, a calix[4]crown ether monolayer was anchored onto a gold surface and then characterized by Fourier Transform infrared reflection absorption spectroscopy, atomic force microscopy, and cyclic voltammetry. The interaction between arginine and its host compound was investigated by SPR. The calix[4]crown ether was found to assemble as a monolayer on the gold surface. Recognition of calix[4]crown monolayer was assessed by the selective binding of arginine. Modification of the SPR chip with the calix[4]crown monolayer provides a reliable and simple experimental platform for investigation of arginine under aqueous conditions.

【 授权许可】

CC BY   
© 2011 by the authors; licensee MDPI, Basel, Switzerland.