A Microfluidic Love-Wave Biosensing Device for PSA Detection Based on an Aptamer Beacon Probe

Sensors

Feng Zhang¹  Shuangming Li¹  Kang Cao¹  Pengjuan Wang²  Yan Su¹  Xinhua Zhu¹  Ying Wan¹ 

[1] School of Mechanical Engineering, Nanjing University of Science and Technology, Nanjing 210094, China; E-Mails:;School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China; E-Mail:

关键词: Love wave;    biosensing;    PSA;    aptamer;    stem-loop probe;    microfluidic;    PDMS;   

DOI  :  10.3390/s150613839

来源: mdpi

【 摘 要 】

A label-free and selective aptamer beacon-based Love-wave biosensing device was developed for prostate specific antigen (PSA) detection. The device consists of the following parts: LiTaO₃ substrate with SiO₂ film as wave guide layer, two set of inter-digital transducers (IDT), gold film for immobilization of the biorecongniton layer and a polydimethylsiloxane (PDMS) microfluidic channels. DNA aptamer, or “artificial antibody”, was used as the specific biorecognition probe for PSA capture. Some nucleotides were added to the 3'-end of the aptamer to form a duplex with the 3'-end, turning the aptamer into an aptamer-beacon. Taking advantage of the selective target-induced assembly changes arising from the “aptamer beacon”, highly selective and specific detection of PSA was achieved. Furthermore, PDMS microfluidic channels were designed and fabricated to realize automated quantitative sample injection. After optimization of the experimental conditions, the established device showed good performance for PSA detection between 10 ng/mL to 1 μg/mL, with a detection limit of 10 ng/mL. The proposed sensor might be a promising alternative for point of care diagnostics.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.