Redox-Active Profile Characterization of Remirea maritima Extracts and Its Cytotoxic Effect in Mouse Fibroblasts (L929) and Melanoma (B16F10) Cells

Molecules

Grace Anne A. Dória⁴  Anderson R. Santos⁴  Leonardo S. Bittencourt³  Rafael C. Bortolin³  Paula P. Menezes⁴  Bruno S. Vasconcelos⁴  Rebeca O. Souza¹  Maria José V. Fonseca¹  Alan Diego C. Santos²  Shanmugam Saravanan⁴  Francilene A. Silva⁴  Daniel P. Gelain³  José Cláudio F. Moreira³  Ana Paula N. Prata⁵  Lucindo J. Quintans-Júnior²  Adriano A. S. Araújo⁴ 

[1] Departament of Pharmacy, University of São Paulo, 14040-900 Ribeirão Preto, São Paulo, Brazil; E-Mails:;Departament of Physiology and Chemistry, Federal University of Sergipe, 49100-000 São Cristóvão, Sergipe, Brazil; E-Mails:;Departament of Biochemistry, Federal University of Rio Grande do Sul, 90040-060 Porto Alegre, Rio Grande do Sul, Brazil; E-Mails:;Departament of Pharmacy, Federal University of Sergipe, Av. Marechal Rondon, Jardim Rosa Elze, 49100-000 São Cristóvão, Sergipe, Brazil; E-Mails:;Departament of Biology, Federal University of Sergipe, 49100-000 São Cristóvão, Sergipe, Brazil; E-Mail:

关键词: Remirea maritima;    free radicals;    cellular viability;    oxidative stress;    cytotoxicity;    total phenol;   

DOI  :  10.3390/molecules200711699

来源: mdpi

【 摘 要 】

Remirea maritima is a tropical plant with a reticulated root system belonging to the family Cyperaceae, also known to have biologically active secondary metabolites. However, very few data on R. maritima’s biological actions are available and there are no reports regarding the redox-active profile of this plant. In this study, we examined the total phenolic content of Remirea maritima hydroalcoholic (RMHA) extracts, redox properties against different reactive species generated in vitro and their cytotoxic effect against fibroblasts (L929) and melanoma (B16F10) cells. Total reactive antioxidant potential index (TRAP) and total antioxidant reactivity (TAR) results revealed that RMHA at all concentrations tested showed significant antioxidant capacity. RMHA was also effective against hydroxyl radical formation, reduction of Fe³⁺ to Fe²⁺ and in scavenging nitric oxide (NO) radicals. In vitro, the level of lipid peroxidation was reduced by RMHA extract and the data showed significant oxidative damage protection. The RMHA cytotoxicity was evaluated by a neutral red assay in fibroblast (L929) and melanome (B16F10) cells. The obtained results showed that the RMHA (40 and 80 µg/mL, respectively) reduced 70% of the viable cells. In conclusion, this study represents the first report regarding the antioxidant and anti-proliferative potential of R. maritima against B16F10 melanoma cells.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.