Pan-Genome Analysis of Brazilian Lineage A Amoebal Mimiviruses

Viruses

Felipe L. Assis¹  Leena Bajrai²  Jonatas S. Abrahao¹  Erna G. Kroon¹  Fabio P. Dornas¹  Kétyllen R. Andrade¹  Paulo V. M. Boratto¹  Mariana R. Pilotto³  Catherine Robert²  Samia Benamar²  Bernard La Scola²  Philippe Colson² 

[1] Instituto de Ciências Biológicas, Departamento de Microbiologia, Laboratório de Vírus, Universidade Federal de Minas Gerais, Belo Horizonte, 31270-901 Minas Gerais, Brazil; E-Mails:;Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE) UM63 CNRS 7278 IRD 198 INSERM U1095, Aix-Marseille Univ., 13385 Marseille, France; E-Mails:;Centro de Ciências Biológicas, Departamento de Microbiologia e Parasitologia, Laboratório de Virologia Aplicada, Universidade Federal de Santa Catarina, Florianópolis, 88040-900 Santa Catarina, Brazil; E-Mail:

关键词: Mimiviridae;    Samba virus;    pan-genome;    mimivirus;    Megavirales;    Amazonia virus;    oyster virus;    Kroon virus;    genomics;   

DOI  :  10.3390/v7072782

来源: mdpi

【 摘 要 】

Since the recent discovery of Samba virus, the first representative of the family Mimiviridae from Brazil, prospecting for mimiviruses has been conducted in different environmental conditions in Brazil. Recently, we isolated using Acanthamoeba sp. three new mimiviruses, all of lineage A of amoebal mimiviruses: Kroon virus from urban lake water; Amazonia virus from the Brazilian Amazon river; and Oyster virus from farmed oysters. The aims of this work were to sequence and analyze the genome of these new Brazilian mimiviruses (mimi-BR) and update the analysis of the Samba virus genome. The genomes of Samba virus, Amazonia virus and Oyster virus were 97%–99% similar, whereas Kroon virus had a low similarity (90%–91%) with other mimi-BR. A total of 3877 proteins encoded by mimi-BR were grouped into 974 orthologous clusters. In addition, we identified three new ORFans in the Kroon virus genome. Additional work is needed to expand our knowledge of the diversity of mimiviruses from Brazil, including if and why among amoebal mimiviruses those of lineage A predominate in the Brazilian environment.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.