Identification and Characterization of 40 Isolated Rehmannia glutinosa MYB Family Genes and Their Expression Profiles in Response to Shading and Continuous Cropping

International Journal of Molecular Sciences

Fengqing Wang²  Yanfei Suo²  He Wei¹  Mingjie Li³  Caixia Xie⁴  Lina Wang⁴  Xinjian Chen²  Zhongyi Zhang² 

[1] Institute of Industrial Crops, Henan Academy of Agricultural Science, Zhengzhou 450002, China; E-Mail:;College of Agronomy, Henan Agricultural University, Zhengzhou 450002, China; E-Mails:;College of Crop Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China; E-Mail:;School of medicine, Henan University of Traditional Chinese Medicine, Zhengzhou 450046, China; E-Mails:

关键词: MYB transcription factor;    shading;    continuous cropping;    Rehmannia glutinosa;   

DOI  :  10.3390/ijms160715009

来源: mdpi

【 摘 要 】

The v-myb avian myeloblastosis viral oncogene homolog (MYB) superfamily constitutes one of the most abundant groups of transcription factors (TFs) described in plants. To date, little is known about the MYB genes in Rehmannia glutinosa. Forty unique MYB genes with full-length cDNA sequences were isolated. These 40 genes were grouped into five categories, one R1R2R3-MYB, four TRFL MYBs, four SMH MYBs, 25 R2R3-MYBs, and six MYB-related members. The MYB DNA-binding domain (DBD) sequence composition was conserved among proteins of the same subgroup. As expected, most of the closely related members in the phylogenetic tree exhibited common motifs. Additionally, the gene structure and motifs of the R. glutinosa MYB genes were analyzed. MYB gene expression was analyzed in the leaf and the tuberous root under two abiotic stress conditions. Expression profiles showed that most R. glutinosa MYB genes were expressed in the leaf and the tuberous root, suggesting that MYB genes are involved in various physiological and developmental processes in R. glutinosa. Seven MYB genes were up-regulated in response to shading in at least one tissue. Two MYB genes showed increased expression and 13 MYB genes showed decreased expression in the tuberous root under continuous cropping. This investigation is the first comprehensive study of the MYB gene family in R. glutinosa.

【 授权许可】

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