Calophyllolide Content in Calophyllum inophyllum at Different Stages of Maturity and Its Osteogenic Activity

Molecules

Wei-Hsien Liu¹  Yen-Wenn Liu²  Zih-Fong Chen³  Wen-Fei Chiou²  Ying-Chieh Tsai¹  Chien-Chih Chen³ 

[1] Institute of Biochemistry and Molecular Biology, National Yang-Ming University, No. 155, Li-Nong St., Sec. 2, Beitou Dist., Taipei 11221, Taiwan; E-Mail:;National Research Institute of Chinese Medicine, No. 155-1, Li-Nong St., Sec. 2, Beitou Dist., Taipei 11221, Taiwan; E-Mails:;Department of Biotechnology, HungKuang University, No. 1018, Sec. 6, Taiwan Boulevard, Shalu District, Taichung City 43302, Taiwan; E-Mail:

关键词: Calophyllum inophyllum;    calophyllolide;    osteoblast;   

DOI  :  10.3390/molecules200712314

来源: mdpi

【 摘 要 】

Calophyllum inophyllum is a coastal plant rich in natural substances. Its ingredients have been used for the development of an anti-human immunodeficiency virus (HIV) drug. In this study, we collected C. inophyllum fruit, and the ethanol extract of the fruit was chromatographically separated using silica gel and Sephadex LH-20 columns to obtain the major compound, calophyllolide. The fruits were harvested from September to December in 2011; a quantitative analysis of the calophyllolide content was conducted using HPLC to explore the differences between the different parts of the fruit during the growing season. The results showed that in fruits of C. inophyllum, calophyllolide exists only in the nuts, and dried nuts contain approximately 2 mg·g⁻¹ of calophyllolide. The calophyllolide levels in the nuts decreased during maturity. In addition, calophyllolide dose-dependently enhanced alkaline phosphatase (ALP) activity in murine osteoblastic MC3T3-E1 cells, without significant cytotoxicity. The expression of osteoblastic genes, ALP and osteocalcin (OCN), were increased by calophyllolide. Calophyllolide induced osteoblasts differentiation also evidenced by increasing mineralization and ALP staining.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.