Mamao Pomace Extract Alleviates Hypertension and Oxidative Stress in Nitric Oxide Deficient Rats

Nutrients

Upa Kukongviriyapan³  Veerapol Kukongviriyapan⁴  Patchareewan Pannangpetch⁴  Wanida Donpunha¹  Jintana Sripui²  Amporn Sae-Eaw² 

[1] Department of Physical Therapy, Faculty of Associated Medical Science, Khon Kaen University, Khon Kaen 40002, Thailand; E-Mail:;Department of Food Technology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand; E-Mails:;Department of Physiology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand;Department of Pharmacology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand; E-Mails:

关键词: Antidesma thwaitesianum;    antioxidant;    endothelial dysfunction;    hypertension;    l-NAME;    Mamao pomace;    nitric oxide;    oxidative stress;   

DOI  :  10.3390/nu7085275

来源: mdpi

【 摘 要 】

Reactive oxygen species (ROS)-induced oxidative stress plays a major role in pathogenesis of hypertension. Antidesma thwaitesianum (local name: Mamao) is a tropical plant distributed in the tropical/subtropical areas of the world, including Thailand. Mamao pomace (MP), a by-product generated from Mamao fruits, contains large amounts of antioxidant polyphenolic compounds. The aim of this study was to investigate the antihypertensive and antioxidative effects of MP using hypertensive rats. For this purpose, male Sprague-Dawley rats were given N^ω-nitro-l-arginine methyl ester (l-NAME), an inhibitor of endothelial nitric oxide synthase (eNOS), in drinking water (50 mg/kg) for three weeks. MP extract was orally administered daily at doses of 100 and 300 mg/kg. l-NAME administration induced marked increase in blood pressure, peripheral vascular resistance, and oxidative stress. MP treatment significantly prevented the increase in blood pressure, hindlimb blood flow and hindlimb vascular resistance of l-NAME treated hypertensive rats (p < 0.05). The antihypertensive effect of MP treatment was associated with suppression of superoxide production from carotid strips and also with an increase in eNOS protein expression and nitric oxide bioavailability. The present results provide evidence for the antihypertensive effect of MP and suggest that MP might be useful as a dietary supplement against hypertension.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.