Conjugation of Polymer-Coated Gold Nanoparticles with Antibodies—Synthesis and Characterization

Nanomaterials

Gamze Tan⁴  Karsten Kantner²  Qian Zhang²  Mahmoud G. Soliman²  Pablo del Pino¹  Wolfgang J. Parak²  Mehmet A. Onur³  Daniel Valdeperez²  Joanna Rejman²  Beatriz Pelaz² 

[1] Centro de Investigación Cooperativa Biomagune, San Sebastián 20001, Spain; E-Mail:;Philipp University of Marburg, Marburg 35001, Germany; E-Mails:;Faculty of Science, Department of Biology, Hacettepe University, Ankara 06800, Turkey; E-Mail:;Faculty of Science and Letters, Department of Biology, Aksaray University, Aksaray 68100, Turkey; E-Mail:

关键词: gold nanoparticles;    bioconjugation;    nanoparticle characterization;    toxicity;    nanoparticle-cell interaction;    cellular uptake;    VEGF;   

DOI  :  10.3390/nano5031297

来源: mdpi

【 摘 要 】

The synthesis of polymer-coated gold nanoparticles with high colloidal stability is described, together with appropriate characterization techniques concerning the colloidal properties of the nanoparticles. Antibodies against vascular endothelial growth factor (VEGF) are conjugated to the surface of the nanoparticles. Antibody attachment is probed by different techniques, giving a guideline about the characterization of such conjugates. The effect of the nanoparticles on human adenocarcinoma alveolar basal epithelial cells (A549) and human umbilical vein endothelial cells (HUVECs) is probed in terms of internalization and viability assays.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.