Lead Poisoning Disturbs Oligodendrocytes Differentiation Involved in Decreased Expression of NCX3 Inducing Intracellular Calcium Overload

International Journal of Molecular Sciences

Teng Ma¹  Xiyan Wu¹  Qiyan Cai¹  Yun Wang¹  Lan Xiao¹  Yanping Tian¹  Hongli Li¹ 

[1] Department of Histology and Embryology, Third Military Medical University, Chongqing 400038, China; E-Mails:

关键词: Pb exposure;    oligodendrocytes;    differentiation;    sodium/calcium exchanger 3;    calcium signal;   

DOI  :  10.3390/ijms160819096

来源: mdpi

【 摘 要 】

Lead (Pb) poisoning has always been a serious health concern, as it permanently damages the central nervous system. Chronic Pb accumulation in the human body disturbs oligodendrocytes (OLs) differentiation, resulting in dysmyelination, but the molecular mechanism remains unknown. In this study, Pb at 1 μM inhibits OLs precursor cells (OPCs) differentiation via decreasing the expression of Olig 2, CNPase proteins in vitro. Moreover, Pb treatment inhibits the sodium/calcium exchanger 3 (NCX3) mRNA expression, one of the major means of calcium (Ca²⁺) extrusion at the plasma membrane during OPCs differentiation. Also addition of KB-R7943, NCX3 inhibitor, to simulate Pb toxicity, resulted in decreased myelin basic protein (MBP) expression and cell branching. Ca²⁺ response trace with Pb and KB-R7943 treatment did not drop down in the same recovery time as the control, which elevated intracellular Ca²⁺ concentration reducing MBP expression. In contrast, over-expression of NCX3 in Pb exposed OPCs displayed significant increase MBP fluorescence signal in positive regions and CNPase expression, which recovered OPCs differentiation to counterbalance Pb toxicity. In conclusion, Pb exposure disturbs OLs differentiation via affecting the function of NCX3 by inducing intracellular calcium overload.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.