Identification of Recurrence-Related microRNAs from Bone Marrow in Hepatocellular Carcinoma Patients

Journal of Clinical Medicine

Keishi Sugimachi³  Shotaro Sakimura³  Akira Tomokuni⁴  Ryutaro Uchi³  Hidenari Hirata³  Hisateru Komatsu³  Yoshiaki Shinden³  Tomohiro Iguchi³  Hidetoshi Eguchi⁴  Takaaki Masuda³  Kazutoyo Morita¹  Ken Shirabe²  Yoshihiko Maehara²  Masaki Mori⁴  Koshi Mimori³  Takahiro Ochiya⁵ 

[1] Department of Surgery, Fukuoka City Hospital, 13-1 Yoshizukahonmachi, Fukuoka 812-0046, Japan; E-Mail:;Department of Surgery and Science Graduate School of Medical Sciences Kyushu University 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan; E-Mails:;Department of Surgery, Kyushu University Beppu Hospital, 4546 Tsurumihara, Beppu 874-0838, Japan; E-Mails:;Department of Gastroenterological Surgery, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan; E-Mails:Department of Surgery, Kyushu University Beppu Hospital, 4546 Tsurumihara, Beppu 874-0838, Japan;

关键词: bone marrow;    microRNA;    hepatocellular carcinoma;    recurrence;   

DOI  :  10.3390/jcm4081600

来源: mdpi

【 摘 要 】

Hepatocellular carcinoma (HCC) is a poor-prognosis cancer due to its high rate of recurrence. microRNAs (miRNAs) are a class of small non-coding RNA molecules that affect crucial processes in cancer development. The objective of this study is to identify the role of miRNAs in patient bone marrow (BM) and explore the function of these molecules during HCC progression. We purified miRNAs from bone marrow cells of seven HCC patients, and divided them into three fractions by cell surface markers as follows: CD14⁺ (macrophage), CD14⁻/CD45⁺ (lymphocyte), and CD14⁻/CD45⁻/EpCAM⁺ (epithelial cell). We employed microarray-based profiling to analyze miRNA expression in the bone marrow of patients with HCC. Differentially expressed miRNAs were significantly different between fractions from whole bone marrow, macrophages, and lymphocytes, and depended on stages in tumor progression. Differences in expression of miRNAs associated with cell proliferation also varied significantly between HCC patients with recurrence, multiple tumors, and advanced clinical stages. These results suggest that miRNA profiles in separated fractions of BM cells are associated with HCC progression.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.