International Journal of Molecular Sciences | |
Avoidance and Potential Remedy Solutions of Chimeras in Reconstructing the Phylogeny of Aphids Using the 16S rRNA Gene of |
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Rui Chen1  Zhe Wang1  Jing Chen1  Ge-Xia Qiao1  | |
[1] Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; E-Mails: | |
关键词:
chimera;
16S rRNA gene;
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DOI : 10.3390/ijms160920152 | |
来源: mdpi | |
【 摘 要 】
It is known that PCR amplification of highly homologous genes from complex DNA mixtures can generate a significant proportion of chimeric sequences. The 16S rRNA gene is not only widely used in estimating the species diversity of endosymbionts in aphids but also used to explore the co-diversification of aphids and their endosymbionts. Thus, chimeric sequences may lead to the discovery of non-existent endosymbiont species and mislead
【 授权许可】
CC BY
© 2015 by the authors; licensee MDPI, Basel, Switzerland.
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