Characterization of Human Gingival Fibroblasts on Zirconia Surfaces Containing Niobium Oxide

Materials

Young-Dan Cho¹  Ji-Cheol Shin⁴  Hyung-In Yoon⁴  Young Ku¹  Hyun-Mo Ryoo⁵  Dae-Joon Kim²  Do-Gyoon Kim³  Jung-Suk Han⁴ 

[1]Department of Periodontology, School of Dentistry, Seoul National University, Seoul 110-749, Korea

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[3]Department of Advanced Materials Engineering, Sejong University, Seoul 143-747, Korea

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[5]Division of Orthodontics, College of Dentistry, Ohio State University, Columbus, OH 43210, USA

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[7]Department of Prosthodontics, School of Dentistry, Seoul National University, Seoul 110-749, Korea

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[9]Department of Molecular Genetics, School of Dentistry and Dental Research Institute, BK21 Program, Seoul National University, Seoul 110-749, Korea

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关键词: dental implant;    zirconia;    niobium;    human gingival fibroblasts (HGFs);    mucosal sealing;   

DOI  :  10.3390/ma8095288

来源: mdpi

【 摘 要 】

It was indicated that tetragonal zirconia polycrystal (TZP) containing yttria (Y₂O₃) and niobium oxide (Nb₂O₅) ((Y,Nb)-TZP) could be an adequate dental material to be used at esthetically important sites. The (Y,Nb)-TZP was also proved to possess its osteogenic potential comparable with those conventional dental implant material, titanium (Ti). The objective of the current study was to characterize cellular response of human gingival fibroblasts (HGFs) to smooth and rough surfaces of the (Y,Nb)-TZP disc, which were obtained by polishing and sandblasting, respectively. Various microscopic, biochemical, and molecular techniques were used to investigate the disc surfaces and cellular responses for the experimental (Y,Nb)-TZP and the comparing Ti groups. Sandblasted rough (Y,Nb)-TZP (Zir-R) discs had the highest surface roughness. HGFs cultured on polished (Y,Nb)-TZP (Zir) showed a rounded cell morphology and light spreading at 6 h after seeding and its proliferation rate significantly increased during seven days of culture compared to other surfaces. The mRNA expressions of type I collagen, integrin α2 and β1 were significantly stimulated for the Zir group at 24 h after seeding. The current findings, combined with the previous results, indicate that (Y,Nb)-TZP provides appropriate surface condition for osseointegration at the fixture level and for peri-implant mucosal sealing at the abutment level producing a suitable candidate for dental implantation with an expected favorable clinical outcome.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.