A Wireless Optogenetic Headstage with Multichannel Electrophysiological Recording Capability

Sensors

Gabriel Gagnon-Turcotte³  Alireza Avakh Kisomi³  Reza Ameli³  Charles-Olivier Dufresne Camaro³  Yoan LeChasseur¹  Jean-Luc Néron¹  Paul Brule Bareil¹  Paul Fortier³  Cyril Bories²  Yves de Koninck²  Benoit Gosselin³ 

[1] Doric Lenses Inc., Quebec, QC G1P 4N7, Canada; E-Mails:;Institut Universitaire en Santé Mentale de Québec, Quebec, QC G1J 2G3, Canada; E-Mails:;Department of Electrical and Computer Engineering, Université Laval, Quebec, QC G1V 0A6, Canada; E-Mails:

关键词: neural headstage;    optogenetics;    neural recording;    optical stimulation;    wireless sensor;    brain-computer interfaces;    low-power biotelemetry;    electrophysiology;   

DOI  :  10.3390/s150922776

来源: mdpi

【 摘 要 】

We present a small and lightweight fully wireless optogenetic headstage capable of optical neural stimulation and electrophysiological recording. The headstage is suitable for conducting experiments with small transgenic rodents, and features two implantable fiber-coupled light-emitting diode (LED) and two electrophysiological recording channels. This system is powered by a small lithium-ion battery and is entirely built using low-cost commercial off-the-shelf components for better flexibility, reduced development time and lower cost. Light stimulation uses customizable stimulation patterns of varying frequency and duty cycle. The optical power that is sourced from the LED is delivered to target light-sensitive neurons using implantable optical fibers, which provide a measured optical power density of 70 mW/mm² at the tip. The headstage is using a novel foldable rigid-flex printed circuit board design, which results into a lightweight and compact device. Recording experiments performed in the cerebral cortex of transgenic ChR2 mice under anesthetized conditions show that the proposed headstage can trigger neuronal activity using optical stimulation, while recording microvolt amplitude electrophysiological signals.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.