Chitosan-Poly (I:C)-PADRE Based Nanoparticles as Delivery Vehicles for Synthetic Peptide Vaccines

Vaccines

Jorge F. Correia-Pinto¹  Noemi Csaba¹  John T. Schiller²  Maria J. Alonso¹ 

[1] Department of Pharmacy and Pharmaceutical Technology, School of Pharmacy, University of Santiago de Compostela, 15782 Santiago de Compostela, Spain; E-Mails:;Laboratory of Cellular Oncology, National Cancer Institute, NIH, Bethesda, MD, USA; E-Mail:

关键词: peptide-based antigens;    nanoparticle;    adjuvant;    chitosan;    poly (I:C);    HPV;    T-Helper peptide;   

DOI  :  10.3390/vaccines3030730

来源: mdpi

【 摘 要 】

The safety and precision of peptide antigens has prompted the search for adjuvants capable of increasing the immune response against these intrinsically poorly immunogenic antigens. The integration of both immunostimulants and peptide antigens within nanometric delivery systems for their co-delivery to immune cells is a promising vaccination strategy. With this in mind, the potential synergistic effect of the immunostimulant poly (I:C) (pIC) and a T-Helper peptide (PADRE), integrated into a chitosan (CS) based nanostructure, was explored. The value of this nanostructured combination of materials was assessed for a peptide antigen (1338aa) derived from the HPV-16 L2 protein. These nanoparticles, produced by ionic gelation technique, exhibited a nanometric size (<300 nm), a high positive surface charge (>40 mV) and high pIC association efficiency (>96%). They also showed capacity for the association of both the 1338aa and PADRE peptides. The influence of the presence of pIC and PADRE in the nanocomposition, as well as that of the peptide presentation form (encapsulated versus surface adsorbed) on the antibody induction was evaluated in a preliminary in vivo study. The data obtained highlights the possibility to engineer nanoparticles through the rational combination of a number of adjuvant molecules together with the antigen.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.