Beneficial Effects of Ethanolic and Hexanic Rice Bran Extract on Mitochondrial Function in PC12 Cells and the Search for Bioactive Components

Molecules

Stephanie Hagl²  Dirk Berressem²  Bastian Bruns²  Nadine Sus¹  Jan Frank¹  Gunter P. Eckert² 

[1] Institute of Biological Chemistry and Nutrition, University of Hohenheim, Stuttgart 70599, Germany; E-Mails:;Department of Pharmacology, Biocenter Campus Riedberg, Goethe-University of Frankfurt, Frankfurt 60438, Germany; E-Mails:

关键词: mitochondria;    rice bran extract;    vitamin E;    tocopherol;    tocotrienol;    PC12 cells;    aging;    prevention;    neurodegeneration;   

DOI  :  10.3390/molecules200916524

来源: mdpi

【 摘 要 】

Mitochondria are involved in the aging processes that ultimately lead to neurodegeneration and the development of Alzheimer’s disease (AD). A healthy lifestyle, including a diet rich in antioxidants and polyphenols, represents one strategy to protect the brain and to prevent neurodegeneration. We recently reported that a stabilized hexanic rice bran extract (RBE) rich in vitamin E and polyphenols (but unsuitable for human consumption) has beneficial effects on mitochondrial function in vitro and in vivo (doi:10.1016/j.phrs.2013.06.008, 10.3233/JAD-132084). To enable the use of RBE as food additive, a stabilized ethanolic extract has been produced. Here, we compare the vitamin E profiles of both extracts and their effects on mitochondrial function (ATP concentrations, mitochondrial membrane potential, mitochondrial respiration and mitochondrial biogenesis) in PC12 cells. We found that vitamin E contents and the effects of both RBE on mitochondrial function were similar. Furthermore, we aimed to identify components responsible for the mitochondria-protective effects of RBE, but could not achieve a conclusive result. α-Tocotrienol and possibly also γ-tocotrienol, α-tocopherol and δ-tocopherol might be involved, but hitherto unknown components of RBE or a synergistic effect of various components might also play a role in mediating RBE’s beneficial effects on mitochondrial function.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.