期刊论文详细信息
Sensors
Enhanced Viability of Endothelial Colony Forming Cells in Fibrin Microbeads for Sensor Vascularization
Jarel K. Gandhi3  Lada Zivkovic3  John P. Fisher2  Mervin C. Yoder1  Eric M. Brey3 
[1] Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, IN 46201, USA; E-Mail:;Fischell Department of Bioengineering, University of Maryland, College Park, MD 20742, USA; E-Mail:;Department of Biomedical Engineering, Wishnick Hall 223, 3255 South Dearborn Street, Chicago, IL 60616, USA; E-Mail:
关键词: tissue engineering;    vascularization;    bioreactor;    long-term sensors;    implantable sensors;    fibrin;   
DOI  :  10.3390/s150923886
来源: mdpi
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【 摘 要 】

Enhanced vascularization at sensor interfaces can improve long-term function. Fibrin, a natural polymer, has shown promise as a biomaterial for sensor coating due to its ability to sustain endothelial cell growth and promote local vascularization. However, the culture of cells, particularly endothelial cells (EC), within 3D scaffolds for more than a few days is challenging due to rapid loss of EC viability. In this manuscript, a robust method for developing fibrin microbead scaffolds for long-term culture of encapsulated ECs is described. Fibrin microbeads are formed using sodium alginate as a structural template. The size, swelling and structural properties of the microbeads were varied with needle gauge and composition and concentration of the pre-gel solution. Endothelial colony-forming cells (ECFCs) were suspended in the fibrin beads and cultured within a perfusion bioreactor system. The perfusion bioreactor enhanced ECFCs viability and genome stability in fibrin beads relative to static culture. Perfusion bioreactors enable 3D culture of ECs within fibrin beads for potential application as a sensor coating.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.

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