Bioassay-Guided Isolation and Identification of Xanthine Oxidase Inhibitory Constituents from the Leaves of Perilla frutescens

Molecules

Li-Na Huo²  Wei Wang²  Chun-Yu Zhang¹  Hai-Bo Shi³  Yang Liu²  Xiao-Hong Liu²  Bing-Hua Guo²  Dong-Mei Zhao²  Hua Gao² 

[1] Health Education Institute of Changchun, Changchun 130021, Jilin, China; E-Mail:;College of Pharmacy, Qingdao University, Qingdao 266021, Shandong, China; E-Mails:;Institute of Changbai Mountain Resources, Jilin Academy of Chinese Medicine Sciences, Changchun 130012, Jilin, China; E-Mail:

关键词: Perilla frutescens (L.) Britt.;    hyperuricemia;    xanthine oxidase;    polyphenols;   

DOI  :  10.3390/molecules201017848

来源: mdpi

【 摘 要 】

Activity-directed fractionation and purification processes were employed to identify xanthine oxidase (XO) inhibitory compounds from the leaves of Perilla frutescens. The total extract was evaluated in vitro on XO inhibitory activity and in vivo in an experimental model with potassium oxonate-induced hyperuricemia in mice which was used to evaluate anti-hyperuricemic activity. The crude extract showed expressive urate-lowering activity results. Solvent partitioning of the total extract followed by macroporous resin column chromatography of the n-butanol extract yielded four extracts and eluted parts. Among them, only the 70% ethanol eluted part of the n-butanol extract showed strong activity and therefore was subjected to separation and purification using various chromatographic techniques. Five compounds showing potent activity were identified by comparing their spectral data with literature values to be caffeic acid, vinyl caffeate, rosmarinic acid, methyl rosmarinate, and apigenin. These results indicate that pending further study, these compounds could be used as novel natural product agents for the treatment of hyperuricemia.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.