A Spectrometric Method for Hydrogen Peroxide Concentration Measurement with a Reusable and Cost-Efficient Sensor

Sensors

Cheng-Chih Hsu¹  Yuan-Rong Lo¹  Yu-Chian Lin¹  Yi-Cen Shi¹  Pang-Lung Li¹ 

关键词: optical sensor;    spectrometry;    hydrogen peroxide;   

DOI  :  10.3390/s151025716

来源: mdpi

【 摘 要 】

In this study we developed a low cost sensor for measuring the concentration of hydrogen peroxide (H₂O₂) in liquids utilizing a spectrometric method. The sensor was tested using various concentrations of a peroxidase enzyme immobilized on a glass substrate. H₂O₂ can be catalyzed by peroxidase and converted into water and oxygen. The reagent 4-amino-phenazone takes up oxygen together with phenol to form a colored product that has absorption peaks at 510 nm and 450 nm. The transmission intensity is strongly related to the hydrogen peroxide concentration, so can be used for quantitative analysis. The measurement range for hydrogen peroxide is from 5 × 10⁻⁵% to 1 × 10⁻³% (0.5 ppm to 10 ppm) and the results show high linearity. This device can achieve a sensitivity and resolution of 41,400 (photon count/%) and 3.49 × 10⁻⁵% (0.35 ppm), respectively. The response time of the sensor is less than 3 min and the sensor can be reused for 10 applications with similar performance.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.