Fully Automated Spectrometric Protocols for Determination of Antioxidant Activity: Advantages and Disadvantages

Molecules

Jiri Sochor¹  Marketa Ryvolova¹  Olga Krystofova¹  Petr Salas¹  Jaromir Hubalek¹  Vojtech Adam¹  Libuse Trnkova¹  Ladislav Havel¹  Miroslava Beklova¹  Josef Zehnalek¹  Ivo Provaznik¹ 

[1] ¹Department of Breeding and Propagation of Horticultural Plants, Faculty of Horticulture, Mendel university in Brno, Valticka 337, CZ-691 44 Lednice, Czech Republic

关键词: antioxidant activity;    spectrometry;    trolox;    gallic acid;   

DOI  :  10.3390/molecules15128618

来源: mdpi

【 摘 要 】

The aim of this study was to describe behaviour, kinetics, time courses and limitations of the six different fully automated spectrometric methods - DPPH, TEAC, FRAP, DMPD, Free Radicals and Blue CrO₅. Absorption curves were measured and absorbance maxima were found. All methods were calibrated using the standard compounds Trolox^® and/or gallic acid. Calibration curves were determined (relative standard deviation was within the range from 1.5 to 2.5 %). The obtained characteristics were compared and discussed. Moreover, the data obtained were applied to optimize and to automate all mentioned protocols. Automatic analyzer allowed us to analyse simultaneously larger set of samples, to decrease the measurement time, to eliminate the errors and to provide data of higher quality in comparison to manual analysis. The total time of analysis for one sample was decreased to 10 min for all six methods. In contrary, the total time of manual spectrometric determination was approximately 120 min. The obtained data provided good correlations between studied methods (R = 0.97 – 0.99).

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.