Simultaneous Determination of Fucoxanthin and Its Deacetylated Metabolite Fucoxanthinol in Rat Plasma by Liquid Chromatography-Tandem Mass Spectrometry

Marine Drugs

Yiping Zhang²  Hao Wu¹  Hongmei Wen¹  Hua Fang²  Zhuan Hong²  Ruizao Yi²  Rui Liu¹  Patrizia Russo³ 

[1] College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, Jiangsu, China; E-Mails:;Third Institute of Oceanophy Sgratate Oceanic Administration, Xiamen 361005, Fujian, China; E-Mails:Third Institute of Oceanophy Sgratate Oceanic Administration, Xiamen 361005, Fujian, China;

关键词: fucoxanthin;    fucoxanthinol;    metabolite;    LC-MS/MS;    pharmacokinetics;   

DOI  :  10.3390/md13106521

来源: mdpi

【 摘 要 】

Fucoxanthin and its deacetylated metabolite fucoxanthinol are two major carotenoids that have been confirmed to possess various pharmacological properties. In the present study, fucoxanthinol was identified as the deacetylated metabolite of fucoxanthin, after intravenous (i.v.) and intragastric gavage (i.g.) administration to rats at doses of 2 and 65 mg/kg, respectively, by liquid chromatography-tandem mass spectrometric (LC-MS/MS) analysis. Next, an accurate and precise LC-MS/MS method was developed to quantitatively determine fucoxanthin and fucoxanthinol in rat plasma. Plasma samples were resolved by LC-MS/MS on a reverse-phase SB-C18 column that was equilibrated and eluted with acetonitrile (A)/aqueous 0.1% formic acid (B; 92/8, v/v) at a flow rate of 0.5 mL/min. Analytes were monitored by multiple-reaction monitoring (MRM) under positive electrospray ionization mode. The precursor/product transitions (m/z) were 659.3→109.0 for fucoxanthin, 617.2→109.0 for fucoxanthinol, and 429.4→313.2 for the internal standard (IS). Calibration curves for fucoxanthin and fucoxanthinol were linear over concentrations ranging from 1.53 to 720 and 1.17 to 600 ng/mL, respectively. The inter- and intraday accuracy and precision were within ±15%. The method was applied successfully in a pharmacokinetic study and the resulting oral fucoxanthin bioavailability calculated.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.