Marine Drugs | |
Simultaneous Determination of |
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Yiping Zhang2  Hao Wu1  Hongmei Wen1  Hua Fang2  Zhuan Hong2  Ruizao Yi2  Rui Liu1  Patrizia Russo3  | |
[1] College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, Jiangsu, China; E-Mails:;Third Institute of Oceanophy Sgratate Oceanic Administration, Xiamen 361005, Fujian, China; E-Mails:Third Institute of Oceanophy Sgratate Oceanic Administration, Xiamen 361005, Fujian, China; | |
关键词: fucoxanthin; fucoxanthinol; metabolite; LC-MS/MS; pharmacokinetics; | |
DOI : 10.3390/md13106521 | |
来源: mdpi | |
【 摘 要 】
Fucoxanthin and its deacetylated metabolite fucoxanthinol are two major carotenoids that have been confirmed to possess various pharmacological properties. In the present study, fucoxanthinol was identified as the deacetylated metabolite of fucoxanthin, after intravenous (i.v.) and intragastric gavage (i.g.) administration to rats at doses of 2 and 65 mg/kg, respectively, by liquid chromatography-tandem mass spectrometric (LC-MS/MS) analysis. Next, an accurate and precise LC-MS/MS method was developed to quantitatively determine fucoxanthin and fucoxanthinol in rat plasma. Plasma samples were resolved by LC-MS/MS on a reverse-phase SB-C18 column that was equilibrated and eluted with acetonitrile (A)/aqueous 0.1% formic acid (B; 92/8,
【 授权许可】
CC BY
© 2015 by the authors; licensee MDPI, Basel, Switzerland.
【 预 览 】
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