The Effect of Interferon-γ and Zoledronate Treatment on Alpha-Tricalcium Phosphate/Collagen Sponge-Mediated Bone-Tissue Engineering

International Journal of Molecular Sciences

Peiqi Li²  Yoshiya Hashimoto¹  Yoshitomo Honda³  Yoshiyuki Arima²  Naoyuki Matsumoto² 

[1] Department of Biomaterials, Osaka Dental University, 8-1 Kuzuha Hanazonocho, Hirakata, Osaka 573-1121, Japan;Department of Orthodontics, Graduate School of Dentistry, Osaka Dental University, 8-1 Kuzuha Hanazonocho, Hirakata, Osaka 573-1121, Japan; E-Mails:;Institute of Dental Research, Osaka Dental University, 8-1 Kuzuha Hanazonocho, Hirakata, Osaka 573-1121, Japan

关键词: bone tissue engineering;    α-TCP/CS;    inflammatory response;    RANKL;    TNF-α;    osteoclastogenesis;    interferon-γ;    zoledronate;   

DOI  :  10.3390/ijms161025678

来源: mdpi

【 摘 要 】

Inflammatory responses are frequently associated with the expression of inflammatory cytokines and severe osteoclastogenesis, which significantly affect the efficacy of biomaterials. Recent findings have suggested that interferon (IFN)-γ and zoledronate (Zol) are effective inhibitors of osteoclastogenesis. However, little is known regarding the utility of IFN-γ and Zol in bone tissue engineering. In this study, we generated rat models by generating critically sized defects in calvarias implanted with an alpha-tricalcium phosphate/collagen sponge (α-TCP/CS). At four weeks post-implantation, the rats were divided into IFN-γ, Zol, and control (no treatment) groups. Compared with the control group, the IFN-γ and Zol groups showed remarkable attenuation of severe osteoclastogenesis, leading to a significant enhancement in bone mass. Histomorphometric data and mRNA expression patterns in IFN-γ and Zol-injected rats reflected high bone-turnover with increased bone formation, a reduction in osteoclast numbers, and tumor necrosis factor-α expression. Our results demonstrated that the administration of IFN-γ and Zol enhanced bone regeneration of α-TCP/CS implants by enhancing bone formation, while hampering excess bone resorption.

【 授权许可】

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.