Interleukins 6 and 15 Levels Are Higher in Subcutaneous Adipose Tissue, but Obesity Is Associated with Their Increased Content in Visceral Fat Depots

International Journal of Molecular Sciences

Marta Izabela Jonas¹  Alina Kurylowicz¹  Zbigniew Bartoszewicz¹  Wojciech Lisik²  Maurycy Jonas²  Zbigniew Wierzbicki²  Andrzej Chmura²  Piotr Pruszczyk³  Monika Puzianowska-Kuznicka¹ 

[1] Department of Human Epigenetics, Mossakowski Medical Research Centre, Polish Academy of Sciences, 5 Pawinskiego Street, 02-106 Warsaw, Poland; E-Mails:;Department of General and Transplantation Surgery, Medical University of Warsaw, 59 Nowogrodzka Street, 02-005 Warsaw, Poland; E-Mails:;Department of Internal Medicine and Cardiology, Medical University of Warsaw, Lindleya 4, 02-005 Warsaw, Poland; E-Mail:

关键词: obesity;    inflammation;    interleukins;    subcutaneous adipose tissue;    visceral adipose tissue;   

DOI  :  10.3390/ijms161025817

来源: mdpi

【 摘 要 】

Excess adiposity is associated with chronic inflammation, which takes part in the development of obesity-related complications. The aim of this study was to establish whether subcutaneous (SAT) or visceral (VAT) adipose tissue plays a major role in synthesis of pro-inflammatory cytokines. Concentrations of interleukins (IL): 1β, 6, 8 and 15 were measured at the protein level by an ELISA-based method and on the mRNA level by real-time PCR in VAT and SAT samples obtained from 49 obese (BMI > 40 kg/m²) and 16 normal-weight (BMI 20–24.9 kg/m²) controls. IL-6 and IL-15 protein concentrations were higher in SAT than in VAT for both obese (p = 0.003 and p < 0.0001, respectively) and control individuals (p = 0.004 and p = 0.001, respectively), while for IL-1β this was observed only in obese subjects (p = 0.047). What characterized obese individuals was the higher expression of IL-6 and IL-15 at the protein level in VAT compared to normal-weight controls (p = 0.047 and p = 0.016, respectively). Additionally, obese individuals with metabolic syndrome had higher IL-1β levels in VAT than did obese individuals without this syndrome (p = 0.003). In conclusion, concentrations of some pro-inflammatory cytokines were higher in SAT than in VAT, but it was the increased pro-inflammatory activity of VAT that was associated with obesity and metabolic syndrome.

【 授权许可】

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.