Reduction of Adipose Tissue Formation by the Controlled Release of BMP-2 Using a Hydroxyapatite-Coated Collagen Carrier System for Sinus-Augmentation/Extraction-Socket Grafting

Materials

Jung-Seok Lee³  Tae-Wan Kim³  Soyon Park²  Byung-Soo Kim¹  Gun-Il Im⁴  Kyoo-Sung Cho³  Chang-Sung Kim³ 

[1] School of Chemical and Biological Engineering, Seoul National University, Seoul 08826, Korea;Department of Applied Life Science, BK21 PLUS Project, College of Dentistry, Yonsei University, Seoul 03722, Korea;Department of Periodontology, Research Institute for Periodontal Regeneration, College of Dentistry, Yonsei University, Seoul 03722, Korea;Department of Orthopaedic Surgery, Ilsan Hospital, Dongguk University, Goyang 10326, Korea;

关键词: bone morphogenetic protein;    collagen;    hydroxyapatite;    drug delivery system;    bone regeneration;   

DOI  :  10.3390/ma8115411

来源: mdpi

【 摘 要 】

The effects of hydroxyapatite (HA)-coating onto collagen carriers for application of recombinant human bone morphogenetic protein 2 (rhBMP-2) on cell differentiation in vitro, and on in vivo healing patterns after sinus-augmentation and alveolar socket-grafting were evaluated. In vitro induction of osteogenic/adipogenic differentiation was compared between the culture media with rhBMP-2 solution and with the released rhBMP-2 from the control collagen and from the HA-coated collagen. Demineralized bovine bone and collagen/HA-coated collagen were grafted with/without rhBMP-2 in sinus-augmentation and tooth-extraction-socket models. Adipogenic induction by rhBMP-2 released from HA-coated collagen was significantly reduced compared to collagen. In the sinus-augmentation model, sites that received rhBMP-2 exhibited large amounts of vascular tissue formation at two weeks and increased adipose tissue formation at eight weeks; this could be significantly reduced by using HA-coated collagen as a carrier for rhBMP-2. In extraction-socket grafting, dimensional reduction of alveolar ridge was significantly decreased at sites received rhBMP-2 compared to control sites, but adipose tissue was increased within the regenerated socket area. In conclusion, HA-coated collagen carrier for Escherichia coli-derived rhBMP-2 (ErhBMP-2) may reduce in vitro induction of adipogenic differentiation and in vivo adipose bone marrow tissue formation in bone tissue engineering by ErhBMP-2.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.