Amelioration of Anemia in the ICGN Mouse, a Renal Anemia Model, with a Subcutaneous Bolus Injection of Erythropoietin Adsorbed to Hydroxyapatite Matrix

Journal of Veterinary Medical Science

Hidetaka SATO⁴  Kosuke HAYASHIDA⁴  Nobuya SASAKI¹  Shin-ichi KATSUDA⁴  Takashi AGUI¹  Minami OHYAMA⁴  Toru TONEGAWA²  Junzo TANAKA²  Ken-ichi NAGASAKI¹  Teppei NAKAMURA⁴  Shigeo ITO³  Toshiyuki IKOMA⁵ 

[1] Laboratory of Laboratory Animal Science and Medicine, Department of Disease Control, Hokkaido University;Department of Metallurgy and Ceramics Science, Tokyo Institute of Technology;Laboratory of Pharmacology, Department of Biomedical Sciences, Graduate School of Veterinary Medicine, Hokkaido University;Section of Biological Safety Research, Chitose Laboratory, Japan Food Research Laboratories;Biomaterials Center, National Institute for Materials Science

关键词: drug delivery system;    erythropoietin;    hydroxyapatite;    ICGN mice;    renal anemia;   

DOI  :  10.1292/jvms.001365

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(34)Cited-By(3)The recombinant human erythropoietin (rhEPO) is used for the treatment of patients with renal anemia. However, rhEPO should be administered subcutaneously or intravenously three times a week. The repetitive injections of rhEPO result in burdens to patients. To resolve this problem, we investigated the sustaining release methods using an rhEPO-hydroxyapatite (HAp) made by spray-drying technique as the drug delivery system. Two types of rhEPO-HAp formulations were prepared; zinc (Zn) formulation and Zn and poly-L-lactic acid (PLA) formulation. These formulations were examined in genetically anemic model, ICGN (ICR-derived glomerulonephritis) mice. According to in vivo release test of rhEPO from HAp in ICGN mice, elevated plasma concentration of rhEPO could be maintained for more than 7 days. These mice showed the amelioration of anemia for more than 3 weeks post-administration without causing any side effect. In conclusion, Zn or Zn/PLA formulation of HAp was considered to be one of the useful carriers of rhEPO for long-term improvement of anemia.

【 授权许可】

Unknown