A Comparative Study on Two Cationic Porphycenes: Photophysical and Antimicrobial Photoinactivation Evaluation

International Journal of Molecular Sciences

Rubén Ruiz-González²  Montserrat Agut²  Elena Reddi¹  Santi Nonell² 

[1] Department of Biology, University of Padova, via U. Bassi 58/B, E-35121 Padova, Italy;Institut Químic de Sarrià, Universitat Ramon Llull, via Augusta 390, I-08017 Barcelona, Spain;

关键词: photodynamic therapy;    antimicrobial photoinactivation;    MRSA;    P. aeruginosa;    photosensitizer;    porphycene;    flow cytometry;    singlet oxygen;   

DOI  :  10.3390/ijms161125999

来源: mdpi

【 摘 要 】

Over the last decades, the number of pathogenic multi-resistant microorganisms has grown dramatically, which has stimulated the search for novel strategies to combat antimicrobial resistance. Antimicrobial photodynamic therapy (aPDT) is one of the promising alternatives to conventional treatments based on antibiotics. Here, we present a comparative study of two aryl tricationic porphycenes where photoinactivation efficiency against model pathogenic microorganisms is correlated to the photophysical behavior of the porphycene derivatives. Moreover, the extent of photosensitizer cell binding to bacteria has been assessed by flow cytometry in experiments with, or without, removing the unbound porphycene from the incubation medium. Results show that the peripheral substituent change do not significantly affect the overall behavior for both tricationic compounds neither in terms of photokilling efficiency, nor in terms of binding.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.