Long-Term Anti-Allodynic Effect of Immediate Pulsed Radiofrequency Modulation through Down-Regulation of Insulin-Like Growth Factor 2 in a Neuropathic Pain Model

International Journal of Molecular Sciences

Chun-Chang Yeh⁴  Hsiao-Lun Sun³  Chi-Jung Huang²  Chih-Shung Wong¹  Chen-Hwan Cherng¹  Billy Keon Huh⁵  Jinn-Shyan Wang³  Chih-Cheng Chien³ 

[1] Department of Anesthesiology and Integrated Pain Management Center, Tri-Service General Hospital and National Defense Medical Center, Taipei 11490, Taiwan;Department of Medical Research, Cathay General Hospital, Taipei 10631, Taiwan;;School of Medicine, Fu Jen Catholic University, New Taipei City 24205, TaiwanDepartment of Chemistry, Fu-Jen Catholic University and Graduate Institute of Basic Medicine, Fu-Jen Catholic University, New Taipei City 24205, Taiwan;Department of Pain Medicine, University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA;

关键词: pulsed radiofrequency;    spared nerve injury;    extra-cellular signal-regulated kinase 1/2;    Insulin-like growth factor II;    gene ontology annotations;   

DOI  :  10.3390/ijms161126013

来源: mdpi

【 摘 要 】

Pulsed radiofrequency (PRF) is effective in the treatment of neuropathic pain in clinical practice. Its application to sites proximal to nerve injury can inhibit the activity of extra-cellular signal-regulated kinase (ERK) for up to 28 days. The spared nerve injury (SNI)+ immPRF group (immediate exposure to PRF for 6 min after SNI) exhibited a greater anti-allodynic effect compared with the control group (SNI alone) or the SNI + postPRF group (application of PRF for 6 min on the 14th day after SNI). Insulin-like growth factor 2 (IGF2) was selected using microarray assays and according to web-based gene ontology annotations in the SNI + immPRF group. An increase in IGF2 and activation of ERK1/2 were attenuated by the immPRF treatment compared with an SNI control group. Using immunofluorescent staining, we detected co-localized phosphorylated ERK1/2 and IGF2 in the dorsal horn regions of rats from the SNI group, where the IGF2 protein predominantly arose in CD11b- or NeuN-positive cells, whereas IGF2 immunoreactivity was not detected in the SNI + immPRF group. Taken together, these results suggest that PRF treatment immediately after nerve injury significantly inhibited the development of neuropathic pain with a lasting effect, most likely through IGF2 down-regulation and the inhibition of ERK1/2 activity primarily in microglial cells.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.