Serum Biomarkers of (Anti)Oxidant Status for Epidemiological Studies

International Journal of Molecular Sciences

Eugène Jansen²  Tatjana Ruskovska¹ 

[1] Faculty of Medical Sciences, Goce Delcev University, 2000 Stip, Macedonia;;Centre for Health Protection, National Institute for Public Health and the Environment, PO Box 1, 3720 BA Bilthoven, The Netherlands

关键词: biomarkers;    nutrition;    vitamins;    oxidative stress;    antioxidant status;    redox status;    epidemiology;   

DOI  :  10.3390/ijms161126032

来源: mdpi

【 摘 要 】

In this review, we disclose a selection of serum/plasma biomarkers of (anti)oxidant status related to nutrition, which can be used for measurements in large-scale epidemiological studies. From personal experience, we have come to the following proposal of a set of biomarkers for nutritional intake, (anti)oxidant status, and redox status. We have selected the individual antioxidant vitamins E and A, and the carotenoids which can be measured in large series by HPLC. In addition, vitamin C was selected, which can be measured by an auto-analyzer or HPLC. As a biomarker for oxidative stress, the ROM assay (reactive oxygen metabolites) was selected; for the redox status, the total thiol assay; and for the total antioxidant status the BAP assay (biological antioxidant potential). All of these biomarkers can be measured in large quantities by an auto-analyzer. Critical points in biomarker validation with respect to blood sampling, storage conditions, and measurements are discussed. With the selected biomarkers, a good set is presented for use in the risk assessment between nutrition and (chronic) diseases in large-scale epidemiological studies. Examples of the successful application of these biomarkers in large international studies are presented.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.