A Liposome-Based Approach to the Integrated Multi-Component Antigen Microarrays

Microarrays

Denong Wang¹ 

[1] Tumor Glycomics Laboratory, SRI International Biosciences Division, Menlo Park, CA 94025, USA; E-Mail

关键词: lipids;    liposome;    liposome microarrays;    antigen microarrays;    natural antibodies;    autoantibodies;    high-throughput serology;    encephalomyelitis;   

DOI  :  10.3390/microarrays4040618

来源: mdpi

【 摘 要 】

This report describes an experimental procedure for constructing integrated lipid, carbohydrate, and protein microarrays. In essence, it prints liposomes on nitrocellulose-coated micro-glass slides, a biochip substrate for spotting protein and carbohydrate microarrays, and the substances that can form liposomes (homo-liposomes) or can be incorporated into liposomes (hetero-liposomes) are suitable for microarray construction using existing microarray spotting devices. Importantly, this technology allows simultaneous detection of serum antibody activities among the three major classes of antigens, i.e., lipids, carbohydrates, and proteins. The potential of this technology is illustrated by its use in revealing a broad-spectrum of pre-existing anti-lipid antibodies in blood circulation and monitoring the epitope spreading of autoantibody reactivities among protein, carbohydrate, and lipid antigens in experimental autoimmune encephalomyelitis (EAE).

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.