Delayed Numerical Chromosome Aberrations in Human Fibroblasts by Low Dose of Radiation

International Journal of Environmental Research and Public Health

Yoon Hee Cho²  Su Young Kim⁴  Hae Dong Woo¹  Yang Jee Kim⁵  Sung Whan Ha³  Hai Won Chung² 

[1] Molecular Epidemiology Branch, Division of Cancer Epidemiology and Prevention, Research Institute, National Cancer Center, Goyang-si, Gyeonggi-do 410-769, Korea;Department of Molecular Epidemiology, School of Public Health, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 151-742, Korea;Department of Radiation Oncology, Seoul National University College of Medicine, 101 Daehak-ro, Jongno-gu, Seoul 110-744, Korea;Department of Preventive Medicine, School of Medicine, Jeju National University, 66 Jejudaehakno, Jeju-si, Jeju-do 690-756, Korea;Da Vinci College of General Education, Chung-ang University, 84 HeukSeok-Ro, DongJak-gu, Seoul 156-756, Korea;

关键词: radiation-induced genomic instability;    X-irradiation;    micronucleus-centromere assay;    micronuclei;    aneuploidy;   

DOI  :  10.3390/ijerph121214979

来源: mdpi

【 摘 要 】

Radiation-induced genomic instability refers to a type of damage transmitted over many generations following irradiation. This delayed impact of radiation exposure may pose a high risk to human health and increases concern over the dose limit of radiation exposure for both the public and radiation workers. Therefore, the development of additional biomarkers is still needed for the detection of delayed responses following low doses of radiation exposure. In this study, we examined the effect of X-irradiation on delayed induction of numerical chromosomal aberrations in normal human fibroblasts irradiated with 20, 50 and 100 cGy of X-rays using the micronucleus-centromere assay. Frequencies of centromere negative- and positive-micronuclei, and aneuploidy of chromosome 1 and 4 were analyzed in the surviving cells at 28, 88 and 240 h after X-irradiation. X-irradiation increased the frequency of micronuclei (MN) in a dose-dependent manner in the cells at all measured time-points, but no significant differences in MN frequency among cell passages were observed. Aneuploid frequency of chromosomes 1 and 4 increased with radiation doses, and a significantly higher frequency of aneuploidy was observed in the surviving cells analyzed at 240 h compared to 28 h. These results indicate that low-dose of X-irradiation can induce delayed aneuploidy of chromosomes 1 and 4 in normal fibroblasts.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.