Parallel Reaction Monitoring: A Targeted Experiment Performed Using High Resolution and High Mass Accuracy Mass Spectrometry

International Journal of Molecular Sciences

Navin Rauniyar¹ 

[1] W.M. Keck Foundation Biotechnology Resource Laboratory, School of Medicine, Yale University, 300 George Street, New Haven, CT 06511, USA;

关键词: parallel reaction monitoring;    PRM;    selected reaction monitoring;    SRM;    proteomics;    mass spectrometry;    targeted;    quantitative proteomics;    Q-Exactive;    high resolution;    accurate mass;   

DOI  :  10.3390/ijms161226120

来源: mdpi

【 摘 要 】

The parallel reaction monitoring (PRM) assay has emerged as an alternative method of targeted quantification. The PRM assay is performed in a high resolution and high mass accuracy mode on a mass spectrometer. This review presents the features that make PRM a highly specific and selective method for targeted quantification using quadrupole-Orbitrap hybrid instruments. In addition, this review discusses the label-based and label-free methods of quantification that can be performed with the targeted approach.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.