In Vivo Effects of Quercetin in Association with Moderate Exercise Training in Improving Streptozotocin-Induced Aortic Tissue Injuries

Molecules

Irina C. Chis¹  Andrei Coseriu³  Ramona Simedrea¹  Adrian Oros²  Andras L. Nagy²  Simona Clichici¹ 

[1]Department of Physiology, “Iuliu Hatieganu” University of Medicine and Pharmacy, Number 1–3, Clinicilor Street, RO-400023 Cluj-Napoca, Romania

[2]Department of Veterinary Toxicology, University of Agricultural Sciences and Veterinary Medicine, Number 3–5, Mănăştur Street, RO-400372 Cluj-Napoca, Romania

[3]Department of Physiology, “Iuliu Haţieganu” University of Medicine and Pharmacy, Number 8, Victor Babes Street, RO-400012 Cluj-Napoca, Romania

关键词: endothelial dysfunction;    diabetes mellitus;    quercetin;    exercise training;   

DOI  :  10.3390/molecules201219802

来源: mdpi

【 摘 要 】

Background: Diabetes mellitus (DM) is a chronic endocrine-metabolic disorder associated with endothelial dysfunction. Hyperglycemia, dyslipidemia and abnormal nitric oxide-mediated vasodilatation are the major causal factors in the development of endothelial dysfunction in DM. The prevention of endothelial dysfunction may be a first target against the appearance of atherosclerosis and cardiovascular diseases. We have investigated the synergistic protective effects of quercetin administration and moderate exercise training on thoracic aorta injuries induced by diabetes. Methods: Diabetic rats that performed exercise training were subjected to a swimming training program (1 h/day, 5 days/week, 4 weeks). The diabetic rats received quercetin (30 mg/kg body weight/day) for 4 weeks. At the end of the study, the thoracic aorta was isolated and divided into two parts; one part was immersed in 10% formalin for histopathological evaluations and the other was frozen for the assessment of oxidative stress markers (malondialdehyde, MDA and protein carbonyls groups, PC), the activity of antioxidant enzymes (superoxide dismutase, SOD and catalase, CAT), nitrite plus nitrate (NOx) production and inducible nitric oxide synthase (iNOS) protein expression. Results: Diabetic rats showed significantly increased MDA and PC levels, NOx production and iNOS expression and a reduction of SOD and CAT activity in aortic tissues. A decrease in the levels of oxidative stress markers, NOx production and iNOS expression associated with elevated activity of antioxidant enzymes in the aortic tissue were observed in quercetin-treated diabetic trained rats. Conclusions: These findings suggest that quercetin administration in association with moderate exercise training reduces vascular complications and tissue injuries induced by diabetes in rat aorta by decreasing oxidative stress and restoring NO bioavailability.

【 授权许可】

CC BY   
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