The Safety Evaluation of Salvianolic Acid B and Ginsenoside Rg1 Combination on Mice

International Journal of Molecular Sciences

Qun Zhao²  Min Yang²  Yanping Deng²  Haitao Yu²  Linlin Wang²  Fukang Teng²  Kenka Cho¹  Hongmei Ma³  Peng Wu²  Xue Li²  Wanying Wu²  Xuan Liu²  Feng Xu⁴  Baohong Jiang²  De-An Guo² 

[1] Takarazuka University of Medical and Health Care, Hanayashiki-Midorigaoka, Takarazuka-City 6660162, Japan;Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Haike Road #501, Shanghai 201203, China;East China University of Science and Technology Shanghai, Meilong Road 130, Shanghai 200237, China;;Shenyang Pharmaceutical University, Wenhua Road #103, Shenyang 110016, China

关键词: safety;    salvianolic acid B;    ginsenoside Rg1;    acute toxicity;    repeated toxicity;   

DOI  :  10.3390/ijms161226176

来源: mdpi

【 摘 要 】

Our previous study indicated that the combination of salvianolic acid B (SalB) and ginsenoside Rg1 (Rg1), the main components of Salvia miltiorrhizae and Panax notoginseng, improves myocardium structure and ventricular function in rats with ischemia/reperfusion injury. The present study aimed to determine the safety of the combined SalB and Rg1 (SalB-Rg1) in mice. The safety of SalB-Rg1 was evaluated through acute toxicity and repeated-dose toxicity. In the acute toxicity study, the up and down procedure was carried out firstly, and then, the Bliss method was applied. In the toxicity study for seven-day repeated treatment of SalB-Rg1, forty Kunming mice were randomly divided into four groups. The intravenous median lethal dose (LD₅₀) of the SalB-Rg1 combination was 1747 mg/kg using the Bliss method. For both the acute toxicity study and the seven-day repeated toxicity study, SalB-Rg1 did not induce significant abnormality on brain, heart, kidney, liver and lung structure at any dose based on H&E stain. There were no significant changes related to the SalB-Rg1 toxicity detected on biochemical parameters for two kinds of toxicity studies. The LD₅₀ in mice was 1747 mg/kg, which was more than one hundred times higher than the effective dose. Both studies of acute toxicity and seven-day repeated dose toxicity indicated the safety of the SalB-Rg1 combination.

【 授权许可】

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