The Safety Evaluation of Salvianolic Acid B and Ginsenoside Rg1 Combination on Mice

International Journal of Molecular Sciences

Hongmei Ma¹  Min Yang²  Xuan Liu²  Peng Wu²  Fukang Teng²  Qun Zhao²  Baohong Jiang²  Wanying Wu²  Haitao Yu²  Linlin Wang²  Yanping Deng²  De-An Guo²  Xue Li²  Feng Xu³  Kenka Cho⁴ 

[1] East China University of Science and Technology Shanghai, Meilong Road 130, Shanghai 200237, China;Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Haike Road 501, Shanghai 201203, China;Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang 110016, China;Takarazuka University of Medical and Health Care, Hanayashiki-Midorigaoka, Takarazuka-City 6660162, Japan;

关键词: safety;    salvianolic acid B;    ginsenoside Rg1;    acute toxicity;    repeated toxicity;   

DOI  :  10.3390/ijms161226176

来源: DOAJ

【 摘 要 】

Our previous study indicated that the combination of salvianolic acid B (SalB) and ginsenoside Rg1 (Rg1), the main components of Salvia miltiorrhizae and Panax notoginseng, improves myocardium structure and ventricular function in rats with ischemia/reperfusion injury. The present study aimed to determine the safety of the combined SalB and Rg1 (SalB-Rg1) in mice. The safety of SalB-Rg1 was evaluated through acute toxicity and repeated-dose toxicity. In the acute toxicity study, the up and down procedure was carried out firstly, and then, the Bliss method was applied. In the toxicity study for seven-day repeated treatment of SalB-Rg1, forty Kunming mice were randomly divided into four groups. The intravenous median lethal dose (LD50) of the SalB-Rg1 combination was 1747 mg/kg using the Bliss method. For both the acute toxicity study and the seven-day repeated toxicity study, SalB-Rg1 did not induce significant abnormality on brain, heart, kidney, liver and lung structure at any dose based on H&E stain. There were no significant changes related to the SalB-Rg1 toxicity detected on biochemical parameters for two kinds of toxicity studies. The LD50 in mice was 1747 mg/kg, which was more than one hundred times higher than the effective dose. Both studies of acute toxicity and seven-day repeated dose toxicity indicated the safety of the SalB-Rg1 combination.

【 授权许可】

Unknown