Limonin, a Component of Dictamni Radicis Cortex, Inhibits Eugenol-Induced Calcium and cAMP Levels and PKA/CREB Signaling Pathway in Non-Neuronal 3T3-L1 Cells

Molecules

Yeo Cho Yoon¹  Sung-Hee Kim¹  Min Jung Kim¹  Hye Jeong Yang¹  Mee-Ra Rhyu¹  Jae-Ho Park¹ 

[1] Division of Functional Food Research, Korea Food Research Institute, 1201-62 Anyangpangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi-do 463-746, Korea;

关键词: limonin;    furanolactone;    cAMP;    olfactory;    calcium;    dictamni radices cortex;   

DOI  :  10.3390/molecules201219840

来源: mdpi

【 摘 要 】

Limonin, one of the major components in dictamni radicis cortex (DRC), has been shown to play various biological roles in cancer, inflammation, and obesity in many different cell types and tissues. Recently, the odorant-induced signal transduction pathway (OST) has gained attention not only because of its function in the perception of smell but also because of its numerous physiological functions in non-neuronal cells. However, little is known about the effects of limonin and DRC on the OST pathway in non-neuronal cells. We investigated odorant-stimulated increases in Ca²⁺ and cAMP, major second messengers in the OST pathway, in non-neuronal 3T3-L1 cells pretreated with limonin and ethanol extracts of DRC. Limonin and the extracts significantly decreased eugenol-induced Ca²⁺ and cAMP levels and upregulated phosphorylation of CREB and PKA. Our results demonstrated that limonin and DRC extract inhibit the OST pathway in non-neuronal cells by modulating Ca²⁺ and cAMP levels and phosphorylation of CREB.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.