International Journal of Environmental Research and Public Health | |
Glyceollin I Reverses Epithelial to Mesenchymal Transition in Letrozole Resistant Breast Cancer through ZEB1 | |
Patrick P. Carriere2  Shawn D. Llopis2  Anna C. Naiki2  Gina Nguyen2  Tina Phan2  Mary M. Nguyen2  Lynez C. Preyan2  Letitia Yearby2  Jamal Pratt2  Hope Burks6  Ian R. Davenport1  Thu A. Nguyen2  KiTani Parker-Lemieux2  Florastina Payton-Stewart3  Christopher C. Williams2  Stephen M. Boué5  Matthew E. Burow6  Bridgette Collins-Burow6  Aaron Hilliard7  A. Michael Davidson7  Syreeta L. Tilghman7  Mark Edberg4  Barbara E. Hayes4  Valerie Montgomery Rice4  | |
[1] Division of Biological and Public Health Sciences, College of Arts and Sciences, Xavier University of Louisiana, 1 Drexel Drive, New Orleans, LA 70125, USA;College of Pharmacy, Xavier University of Louisiana, 1 Drexel Drive, New Orleans, LA 70125, USA;Division of Mathematical and Physical Sciences, College of Arts and Sciences, Xavier University of Louisiana, 1 Drexel Drive, New Orleans, LA 70125, USA;;College of Pharmacy, Xavier University of Louisiana, 1 Drexel Drive, New Orleans, LA 70125, USASouthern Regional Research Center, United States Department of Agriculture, New Orleans, LA 70124, USA;Tulane University Health Sciences Center, 1430 Tulane Ave, New Orleans, LA 70112, USA;Division of Basic Sciences, College of Pharmacy and Pharmaceutical Sciences, Florida A&M University, 1415 S. Martin L. King Jr. Blvd., Tallahassee, FL 32307, USA; | |
关键词: letrozole resistance; epithelial mesenchymal transition; breast cancer; phytochemicals; aromatase inhibitors; metastasis; | |
DOI : 10.3390/ijerph13010010 | |
来源: mdpi | |
【 摘 要 】
Although aromatase inhibitors are standard endocrine therapy for postmenopausal women with early-stage metastatic estrogen-dependent breast cancer, they are limited by the development of drug resistance. A better understanding of this process is critical towards designing novel strategies for disease management. Previously, we demonstrated a global proteomic signature of letrozole-resistance associated with hormone-independence, enhanced cell motility and implications of epithelial mesenchymal transition (EMT). Letrozole-resistant breast cancer cells (LTLT-Ca) were treated with a novel phytoalexin, glyceollin I, and exhibited morphological characteristics synonymous with an epithelial phenotype and decreased proliferation. Letrozole-resistance increased Zinc Finger E-Box Binding Homeobox 1 (ZEB1) expression (4.51-fold), while glyceollin I treatment caused a −3.39-fold reduction. Immunofluorescence analyses resulted of glyceollin I-induced increase and decrease in E-cadherin and ZEB1, respectively.
【 授权许可】
CC BY
© 2015 by the authors; licensee MDPI, Basel, Switzerland.
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