A Herbal Formula, Atofreellage, Ameliorates Atopic Dermatitis-Like Skin Lesions in an NC/Nga Mouse Model

Molecules

Won-Yong Kim¹  Hyeong-Geug Kim¹  Hye-Won Lee²  Jin-Seok Lee¹  Hwi-Jin Im¹  Hyo-Seon Kim¹  Sung-Bae Lee¹  Chang-Gue Son¹ 

[1] Liver and Immunology Research-Center, Daejeon Oriental Hospital of Daejeon University, 176-9, Daeheung-ro, Jung-Gu, Daejeon 34929, Korea;TKM-Based Herbal Drug Research Group, Korea Institute of Oriental Medicine, Daejeon 34054, Korea;

关键词: atopic dermatitis;    herbal medicine;    inflammation;    NC/Nga murine model;   

DOI  :  10.3390/molecules21010035

来源: mdpi

【 摘 要 】

We evaluated the anti-atopic dermatitis (AD) effect of Atofreellage (AF), a herbal formula composed of 10 medicinal plants. AD was induced on the dorsal skin areas of NC/Nga mice (male, seven weeks old) by daily application of 2,4-dinitrochlorobenzene (DNCB) for five weeks. After three weeks of DNCB application, 200 μL of AF (0, 25, 50 or 100 mg/mL) was applied to the skin lesions. Histological findings, blood cell populations, serum levels of immunoglobulin E (IgE), histamine, pro-inflammatory cytokines, and inflammatory signaling in the skin tissue, and T-helper cell type 2 (Th₂)-related cytokines in splenocytes were analyzed. Histopathological findings showed AF treatment notably attenuated the thickness of dorsal skin, and eosinophil infiltration. AF treatment (especially 100 mg/mL) also demonstrably ameliorated the blood cell population abnormalities, as the notable elevation of serum concentrations of IgE, histamine, TNF-α, IL-6 and IL-1β were remarkably normalized by AF treatment. Western blot analysis evidenced the apparent normalization of inflammatory signals (ERK, p38 MAP kinase, JNK, and NF-κB) in the skin tissue. Additionally, AF treatment notably attenuated the activation of Th₂-dominant cytokines (IL-13, IL-4, and IL-5) in Con A-treated splenocytes in an ex vivo assay. In conclusion, this study provides experimental evidence for the clinical relevance of Atofreellage.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.