Polyphenolic Composition and Evaluation of Antioxidant Activity, Osmotic Fragility and Cytotoxic Effects of Raphiodon echinus (Nees & Mart.) Schauer

Molecules

Antonia Eliene Duarte⁴  Emily Pansera Waczuk¹  Katiane Roversi³  Maria Arlene Pessoa da Silva⁷  Luiz Marivando Barros⁴  Francisco Assis Bezerra da Cunha⁴  Irwin Rose Alencar de Menezes²  José Galberto Martins da Costa⁵  Aline Augusti Boligon⁶  Adedayo Oluwaseun Ademiluyi¹  Jean Paul Kamdem¹  João Batista Teixeira Rocha¹  Marilise Escobar Burger³ 

[1] Programa de Pós-Graduação em Bioquímica Toxicológica, Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Santa Maria, Santa Maria 97105-900, RS, Brazil;Laboratório de Farmacologia e Química Molecular, Departamento de Química Biológica, Universidade Regional do Cariri, Pimenta, Crato CEP 63.100-000, CE, Brazil;Departamento de Fisiologia e Farmacologia, Universidade Federal de Santa Maria, Santa Maria 97105-900, RS, Brazil;Centro de Ciências Biológicas e da Saúde-CCBS, Departamento de Ciências Biológicas, Universidade Regional do Cariri (URCA), Pimenta, Crato CEP 63.100-000, CE, Brazil;Laboratório de Pesquisas de Produtos Naturais, Departamento de Química Biológica, Universidade Regional do Cariri, Crato CEP 63.105.000, CE, Brazil;Laboratório de Fitoquímica, Departamento de Farmácia Industrial, Universidade Federal de Santa Maria, Santa Maria 97105-900, RS, Brazil;Laboratório de Botânica Aplicada, Departamento de Ciências Biológicas, Universidade Regional do Cariri (URCA), Pimenta, Crato CEP 63.100-000, CE, Brazil;

关键词: Raphiodon echinus;    antioxidant activity;    phenolic acids;    HPLC-DAD;   

DOI  :  10.3390/molecules21010002

来源: mdpi

【 摘 要 】

Raphiodon echinus (R. echinus) is used in Brazilian folk medicine for the treatment of inflammation, coughs, and infectious diseases. However, no information is available on the potential antioxidant, cytotoxicity and genotoxicity of this plant. In this study, the polyphenolic constituents, antioxidant capacity and potential toxic effects of aqueous and ethanolic extracts of R. echinus on human erythrocytes and leukocytes were investigated for the first time. R. echinus extracts showed the presence of Gallic, chlorogenic, caffeic and ellagic acids, rutin, quercitrin and quercetin. Aqueous and ethanolic extracts of R. echinus exhibited antioxidant activity in DPPH radical scavenging with IC₅₀ = 111.9 μg/mL (EtOH extract) and IC₅₀ = 227.9 μg/mL (aqueous extract). The extracts inhibited Fe²⁺ (10 μM) induced thiobarbituric acid reactive substances (TBARS) formation in rat brain and liver homogenates. The extracts (30–480 μg/mL) did not induce genotoxicity, cytotoxicity or osmotic fragility in human blood cells. The findings of this present study therefore suggest that the therapeutic effect of R. echinus may be, in part, related to its antioxidant potential. Nevertheless, further in vitro and in vivo studies are required to ascertain the safety margin of its use in folk medicine.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.