Plasma Free Amino Acid Responses to Intraduodenal Whey Protein, and Relationships with Insulin, Glucagon-Like Peptide-1 and Energy Intake in Lean Healthy Men

Nutrients

Natalie D. Luscombe-Marsh¹  Amy T. Hutchison¹  Stijn Soenen¹  Robert E. Steinert¹  Peter M. Clifton¹  Michael Horowitz¹ 

[1] NHMRC Centre of Research Excellence in Translating Nutritional Science to Good Health, The University of Adelaide Discipline of Medicine, Adelaide 5000, Australia;

关键词: dairy;    whey protein hydrolysate;    insulinotropic response;    glycemic control;    appetite regulation;    human;   

DOI  :  10.3390/nu8010004

来源: mdpi

【 摘 要 】

This study determined the effects of increasing loads of intraduodenal (ID) dairy protein on plasma amino acid (AA) concentrations, and their relationships with serum insulin, plasma glucagon-like peptide-1 (GLP-1) and energy intake. Sixteen healthy men had concentrations of AAs, GLP-1 and insulin measured in response to 60-min ID infusions of hydrolysed whey protein administered, in double-blinded and randomised order, at 2.1 (P2.1), 6.3 (P6.3) or 12.5 (P12.5) kJ/min (encompassing the range of nutrient emptying from the stomach), or saline control (C). Energy intake was quantified immediately afterwards. Compared with C, the concentrations of 19/20 AAs, the exception being cysteine, were increased, and this was dependent on the protein load. The relationship between AA concentrations in the infusions and the area under the curve from 0 to 60 min (AUC_{0–60 min}) of each AA profile was strong for essential AAs (R² range, 0.61–0.67), but more variable for non-essential (0.02–0.54) and conditional (0.006–0.64) AAs. The AUC_{0–60 min} for each AA was correlated directly with the AUC_{0–60 min} of insulin (R² range 0.3–0.6), GLP-1 (0.2–0.6) and energy intake (0.09–0.3) (p < 0.05, for all), with the strongest correlations being for branched-chain AAs, lysine, methionine and tyrosine. These findings indicate that ID whey protein infused at loads encompassing the normal range of gastric emptying increases plasma concentrations of 19/20 AAs in a load-dependent manner, and provide novel information on the close relationships between the essential AAs, leucine, valine, isoleucine, lysine, methionine, and the conditionally-essential AA, tyrosine, with energy intake, insulin and GLP-1.

【 授权许可】

CC BY   
© 2016 by the authors; licensee MDPI, Basel, Switzerland.