Neuropeptide Substance-P-Conjugated Chitosan Nanofibers as an Active Modulator of Stem Cell Recruiting

International Journal of Molecular Sciences

Min Sup Kim²  Sang Jun Park²  Wheemoon Cho²  Bon Kang Gu²  Chun-Ho Kim¹  Hitoshi Sashiwa²  Qinghua Qin² 

[1] Laboratory of Tissue Engineering, Korea Institute of Radiological and Medical Science, Seoul 139240, Korea

关键词: chitosan;    substance-P;    wound healing;    electrospinning;    human mesenchymal stem cell;    tissue engineering;   

DOI  :  10.3390/ijms17010068

来源: mdpi

【 摘 要 】

The goal to successful wound healing is essentially to immobilize and recruit appropriate numbers of host stem or progenitor cells to the wound area. In this study, we developed a chitosan nanofiber-immobilized neuropeptide substance-P (SP), which mediates stem cell mobilization and migration, onto the surfaces of nanofibers using a peptide-coupling agent, and evaluated its biological effects on stem cells. The amount of immobilized SP on chitosan nanofibers was modulated over the range of 5.89 ± 3.27 to 75.29 ± 24.31 ng when reacted with 10 to 500 ng SP. In vitro migration assays showed that SP-incorporated nanofibers induced more rapid migration of human mesenchymal stem cells on nanofibers compared to pristine samples. Finally, the conjugated SP evoked a minimal foreign body reaction and recruited a larger number of CD29- and CD44-positive stem cells into nanofibers in a mouse subcutaneous pocket model.

【 授权许可】

CC BY   
© 2016 by the authors; licensee MDPI, Basel, Switzerland.