Surface Functional Poly(lactic Acid) Electrospun Nanofibers for Biosensor Applications

Materials

Edurne González¹  Larissa M. Shepherd¹  Laura Saunders²  Margaret W. Frey¹ 

[1] Department of Fiber Science and Apparel Design, Cornell University, Ithaca, NY 14853, USA;Chemical and Biological Engineering Department, University at Buffalo, Buffalo, NY 14261, USA;

关键词: biotin;    avidin;    poly(lactic acid) PLA;    poly(lactic acid)-block-poly(ethylene glycol) (PLA-b-PEG);    electrospinning;    functional nanofibers;   

DOI  :  10.3390/ma9010047

来源: mdpi

【 摘 要 】

In this work, biotin surface functionalized hydrophilic non-water-soluble biocompatible poly(lactic acid) (PLA) nanofibers are created for their potential use as biosensors. Varying concentrations of biotin (up to 18 weight total percent (wt %)) were incorporated into PLA fibers together with poly(lactic acid)-block-poly(ethylene glycol) (PLA-b-PEG) block polymers. While biotin provided surface functionalization, PLA-b-PEG provided hydrophilicity to the final fibers. Morphology and surface-available biotin of the final fibers were studied by Field Emission Scanning Electron Microscopy (FESEM) and competitive colorimetric assays. The incorporation of PLA-b-PEG block copolymers not only decreased fiber diameters but also dramatically increased the amount of biotin available at the fiber surface able to bind avidin. Finally, fiber water stability tests revealed that both biotin and PLA-b-PEG, migrated to the aqueous phase after relatively extended periods of water exposure. The functional hydrophilic nanofiber created in this work shows a potential application as a biosensor for point-of-care diagnostics.

【 授权许可】

CC BY   
© 2016 by the authors; licensee MDPI, Basel, Switzerland.