DREF is involved in the steroidogenesis via regulation of shadow gene

American Journal of Cancer Research

Yoon-Jeong Choi¹  Young-Shin Kim¹  Masamitsu Yamaguchi¹  Joung-Sun Park¹  Mi-Ae Yoo¹  Dang Thi Phuong Thao¹ 

关键词: Drosophila;    DRE;    DREF;    transcriptional regulation;    ecdysone;    shadow;    steroidogenesis;   

DOI  :  

学科分类：肿瘤学

来源: e-Century Publishing Corporation

【 摘 要 】

The Drosophila DNA replication-related element-binding factor (dDREF) has been identified as a master regulator of cell proliferation-related genes via its binding to the DRE sequence, 5′-TATCGATA. However, the biological roles of DREF are still to be clarified. Here, we show that DREF mutant females have steroid hormone ecdysone-deficient phenotypes, such as the loss of vitellogenic egg chambers. Furthermore, DREF knockdown in the prothoracic gland of larva prevented pupation and this was rescued via 20-hydroxyecdysone treatment. We found a DRE-like sequence (-625 to -632) in the 5′-flanking region of the Drosophila shadow gene, which catalyzes the conversion of 2-deoxyecdysone to ecdysone, and demonstrated that shadow is a novel target gene of dDREF using quantitative RT-PCR and Chip assays. In addition, we show that the level of dDREF protein correlated with age-related changes in the level of shadow mRNA in the ovaries of wild-type flies. Taken together, our data indicate that dDREF plays a key role in steroid synthesis via regulation of the shadow gene.

【 授权许可】

Unknown